DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BENZOXONIUM CHLORIDE AND LIDOCAINE HYDROCHLORIDE
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2017, Vol 3, Issue 5
Abstract
The aim of the present study was to develop and validate reverse phase high performance liquid chromatography (RP-HPLC) method for simultaneous estimation of Benzoxonium chloride and Lidocaine Hydrochloride in bulk and combined dosage form. An isocratic, RP-HPLC method was developed on Nucleosil C18 (250 x 4.6 mm, 5 μm) column using 10 mM potassium dihydrogen phosphate buffer and acetonitritle (20:80 v/v) as mobile phase at flow rate of 1 ml/min at detection wavelength of 215 nm. The chromatographic conditions yield good separation between drugs with retention time (RT) of 5.28 ± 0.13 min and 9.76 ± 0.36 min for Lidocaine hydrochloride and Benzoxonium chloride, respectively. The method was validated with respect to linearity, precision, accuracy and robustness. The data of linear regression analysis indicated a good linear relationship over the range of 20-120 μg/ml for both drugs with a correlation coefficient (r2) of 0.998 for Benzoxonium Chloride and 0.996 for Lidocaine hydrochloride. The developed method was found to be simple, sensitive, selective, accurate and repeatable for simultaneous analysis of Benzoxonium chloride and Lidocaine hydrochloride and can be adopted for routine analysis of these drugs in bulk and pharmaceutical dosage form.
Authors and Affiliations
Dr. Santosh V. Gandhi
Keywords
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