Development and validation of RP-HPLC method for simultaneous estimation of Ertugliflozin and Sitagliptin in bulk drug and tablet dosage form

Journal Title: International Journal of Experimental Research and Review - Year 2023, Vol 32, Issue 3

Abstract

To treat type 2 diabetes, in a combined tablet dosage form the ertugliflozin and sitagliptin were administered. Considering the less complication and readily availability of HPLC, the main objective of present study was to develop a new, precise, accurate, linear, robust, and economical RP-HPLC method for the simultaneous estimation of ertugliflozin and sitagliptin in tablet dosage form. Effective chromatographic separation of Ertugliflozin and Sitagliptin was achieved on Kromasil C18 (5 µm 250 mm X 4.6 mm) and the mobile phase containing Methanol and 0.1% OPA in water isocratic elution mode at a flow rate of 1.0mL/min. with column temperature at 30 °C and the injection volume was 20 µL at column temperature at 30°C. At an isosbestic wavelength of 212 nm, ertugliflozin and sitagliptin were found to have retention times of 5.30 min. and 2.05 min., respectively. The method was proven to be precise (%RSD 2%), accurate (>90%), and specific for the simultaneous measurement of both drugs in tablets. As a result, the suggested method with excellent specificity, accuracy, precision, linearity and robustness as well as economical was useful for the regular quality control analysis of ertugliflozin and sitagliptin tablets.

Authors and Affiliations

S. D. Mankar, Priyanka Sagar, Sanjay Bhawar, Suhas Siddheshwar, Santosh Dighe

Keywords

Related Articles

Artificial Intelligence Driven Bibliometric Insights: Pioneering Down Syndrome Research

The present bibliometric analysis investigates the scholarly output from 2013 to 2022 to explore the use of artificial intelligence (AI) in Down syndrome research. The analysis demonstrates a significant and rapid growth...

Liquid bio-slurry enhances the productivity of N-fertilized maize under field conditions in Ethiopia

Enhancing maize production and productivity is critical for ensuring small-scale farmers' food security. Yet, declining soil fertility poses a substantial challenge to cereal production, including maize, in developing co...

Optimizing User Satisfaction in Movie Recommendations Using Variable Learning Rates and Dynamic Neighborhood Functions in SOMs

Customized movie recommendations are crucial in elevating user satisfaction and engagement in the era of vast online entertainment options. This study presents an innovative approach utilizing Enhanced Self-Organizing Ma...

Formulation and Evaluation of Polyherbal Emulgel

The foible of allopathic medicines has resulted in adopting herbal plants that have been proven cost-effective with fewer adverse effects. Thus, the evaluation of the potential of herbal plants for the formulation of inn...

Water bird diversity in Winter and Summer season of Motijheel lake, Murshidabad, West Bengal, India

Wetlands are the most preferred habitats for water birds. They support the birds by way of feeding, roosting, nesting and rearing young ones. Birds are essential animal group of an ecosystem and maintain a trophic level....

Download PDF file
  • EP ID EP720675
  • DOI 10.52756/ijerr.2023.v32.025
  • Views 49
  • Downloads 0

How To Cite

S. D. Mankar, Priyanka Sagar, Sanjay Bhawar, Suhas Siddheshwar, Santosh Dighe (2023). Development and validation of RP-HPLC method for simultaneous estimation of Ertugliflozin and Sitagliptin in bulk drug and tablet dosage form. International Journal of Experimental Research and Review, 32(3), -. https://europub.co.uk/articles/-A-720675