Development and validation of RP-HPLC method for simultaneous estimation of Ertugliflozin and Sitagliptin in bulk drug and tablet dosage form
Journal Title: International Journal of Experimental Research and Review - Year 2023, Vol 32, Issue 3
Abstract
To treat type 2 diabetes, in a combined tablet dosage form the ertugliflozin and sitagliptin were administered. Considering the less complication and readily availability of HPLC, the main objective of present study was to develop a new, precise, accurate, linear, robust, and economical RP-HPLC method for the simultaneous estimation of ertugliflozin and sitagliptin in tablet dosage form. Effective chromatographic separation of Ertugliflozin and Sitagliptin was achieved on Kromasil C18 (5 µm 250 mm X 4.6 mm) and the mobile phase containing Methanol and 0.1% OPA in water isocratic elution mode at a flow rate of 1.0mL/min. with column temperature at 30 °C and the injection volume was 20 µL at column temperature at 30°C. At an isosbestic wavelength of 212 nm, ertugliflozin and sitagliptin were found to have retention times of 5.30 min. and 2.05 min., respectively. The method was proven to be precise (%RSD 2%), accurate (>90%), and specific for the simultaneous measurement of both drugs in tablets. As a result, the suggested method with excellent specificity, accuracy, precision, linearity and robustness as well as economical was useful for the regular quality control analysis of ertugliflozin and sitagliptin tablets.
Authors and Affiliations
S. D. Mankar, Priyanka Sagar, Sanjay Bhawar, Suhas Siddheshwar, Santosh Dighe
Keywords
Related Articles
- EP ID EP720675
- DOI 10.52756/ijerr.2023.v32.025
- Views 45
- Downloads 0
Facial Expression Analysis Using Multi-label Classification
Emotions form the reflection of an individual's mental state. Automatic expression analysis and its recognition based on their facial landmarks are of critical use, particularly to understand the emotional states of phys...
Methanol extract of Isodon ternifolius (D. Don) KUDO leaves has antimicrobial and antioxidant activities but no neuroprotective activity.
Isodon ternifolius (D. Don) KUDO is a medicinal plant traditionally used by the indigenous people of Manipur, India, as a room fumigant and a means to ward off evil spirits. The current study was designed to assess the a...
Stability indicating HPLC method development and validation of Fostemsavir in bulk and marketed formulations by implementing QbD approach
Background: Achieving a predictable degree of quality with intended and planned specifications is known as "quality by design." QbD (Quality-by-Design) is an alternative to conventional method development that places mor...
Providing Highest Privacy Preservation Scenario for Achieving Privacy in Confidential Data
Machine learning algorithms have been extensively employed in multiple domains, presenting an opportunity to enable privacy. However, their effectiveness is dependent on enormous data volumes and high computational resou...
Review on N-confused porphyrin
We all know how vital porphyrin is to our body, from binding haemoglobin and iron to carrying oxygen to our organs and tissues. Recently, there has been a discovery about a new isomer of porphyrin called N-confused porph...