DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE DETERMINATION OF DASATINIB IN TABLET DOSAGE FORM
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 10
Abstract
The objective of the present study was to develop and validate a novel RP-HPLC method for the determination of Dasatinib in the pharmaceutical dosage form. Chromatographic separation was conducted on agilent technologies-1260 series with the G1311C quaternary pump, Thermo Scientific C18 column (4.6 mm i.d. × 250 mm, 5 µm particle size) and equipped with photodiode array detector G1315D. The mobile phase consisted of methanol and acetonitrile mixed in the ratio of 50:50 v/v, was used at a flow rate of 1 ml/min, and the detection wavelength was set at 323 nm. The retention time for Dasatinib was found to be 4.073 min. The calibration was linear (r2 = 0.999) in the concentration range of 2-10 µg/ml. The limit of detection and the limit of quantitation were found to be 0.5263 μg/ml and 1.5948 µg/ml, respectively. Recovery of Dasatinib in tablet formulation was observed in the range of 98.09-99.57%. Percentage assay of Dasatinib (Dasanat) was found to be 99.45% w/w. Thus the novel proposed method for Dasatinib was found to be feasible for the estimation of Dasatinib in bulk as well as the pharmaceutical dosage form.
Authors and Affiliations
P. R. Sankar et al.
LOW COST AND RAPID BIOSYNTHESIS OF SILVER NANOPARTICLES USING CASSIA TORA LEAF EXTRACT
Chemical, physical, and biological methods have been developed to synthesis nanoparticles but chemical and physical methods are involved in the production of toxic by-products which are hazardous moreover the methods are...
VALIDATED KINETIC SPECTROPHOTOMETRIC DETERMINATION OF DRUGS USING ALKALINE POTASSIUM PERMANGANATE OXIDATION
A simple and sensitive kinetic method is described for the determination of drugs viz., Bendamustine hydrochloride (BEN), Ciprofloxacin hydrochloride (CIP), Dorzolamide hydrochloride (DOR), Pantoprazole sodium (PAN), War...
DETERMINATION OF AMINO ACIDS IN TWO SPECIES OF RICCIA
Two species of Riccia, namely Riccia billardieri and Riccia discolor, were studied to detect various amino acids qualitatively as well as quantitatively through HPLC. Seven amino acids (histidine, serine, tryptophan, asp...
INDIRECT ORGANOGENESIS OF CLEOME GYNANDRA L. USING LEAF EXPLANTS – AN IMPORTANT MEDICINAL PLANT
The present study was developed a suitable protocol for callus induction and plant regeneration of Cleome gynandra L. from leaf explants. Callus was initiated from leaf explants on Murashige and Skoog (MS) medium supplem...
ISOLATION AND CHARACTERIZATION OF L-ASPARAGINASE EXTRACELLULAR ENZYME PRODUCING BACTERIA FROM INDUSTRIAL SOIL SAMPLES
Industrial soil contains a variety of L-Asparaginase producing microorganisms such as bacteria and fungi. Microorganisms can produce different types of enzymes which are frequently used in different types of industries f...