Development and Validation of RP – HPLC Method for the estimation of Oxyclozanide in Pure and Pharmaceutical formulation
Journal Title: International Journal of Pharmacy and Analytical Research - Year 2014, Vol 3, Issue 2
Abstract
A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of oxyclozanide from pharmaceutical formulation. Chromatographic separation was achieved gradient on a YMC c18 column (250 x 4.6 mm, 5 µ particle size) using a mobile phase acetonitrile and water in the ratio of 80:20.the flow rate was 1.0ml / min and effluent was detected at 300nm.the retention time of oxyclozanide was found to be 1.89min. Linearity was observed in the concentration range of 10 -100µg / ml .The method was validated according to ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of oxyclozanide.
Authors and Affiliations
Anusha G
Keywords
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