Development and validation of RP – HPLC method for the estimation of Tylosin tartrate in pure and pharmaceutical formulation
Journal Title: International Journal of Pharmacy and Analytical Research - Year 2014, Vol 3, Issue 2
Abstract
A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of tylosin tartrate from pharmaceutical formulation. Chromatographic separation was achieved gradient on a phenomenex c18 column (250 x 4.6 mm, 5 µ particle size) using a mobile phase. Acetonitrile and water in the ratio of 90:10.the flow rate was 1.5ml / min and effluent was detected at 292 nm. The retention time of tylosin tartrate was found to be 2min. linearity was observed in the concentration range of 50 -250µg /ml. The method was validated according to ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of tylosin tartrate.
Authors and Affiliations
Sailaja Kotha
Keywords
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- EP ID EP656120
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