DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR THE SIMULTANEOUS ESTIMATION OF MICONAZOLE NITRATE AND METRONIDAZOLE IN BULK POWDER FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 7
Abstract
Simple, precise, accurate and economical spectrophotometric methods have been developed for simultaneous determination of Miconazole nitrate (MIZ) and Metronidazole (MNZ) in their bulk powder form. Method (I) is simultaneous equation method and method (II) is absorbance ratio method. This is based on measurement of absorbance at two wavelengths 318.8 nm and 279 nm, λmax of Metronidazole and Miconazole nitrate in pH 4.0 phosphate buffers. Both the drugs obeyed Beer-Lambert’s law in the concentration range of 5-30 µg/mL for Metronidazole and 100-600 µg/mL for Miconazole nitrate. The high values of correlation coefficient (R2) indicated good linearity of calibration curve for both the drugs. The second method i.e. absorbance ratio method which involves the formation of Q-absorbance equation using the respective absorptivity values at 283 nm (isoabsorptive point) and 279 nm (λmax of miconazole nitrate); accuracy, precision and recovery studies of the developed methods have been carried out in order to confirm their accuracy.
Authors and Affiliations
Dhanashree Mundhey , Princy Morris , Govind Lohiya , Jasmine Avari
Keywords
Related Articles
- EP ID EP116270
- DOI 10.7897/2230-8407.0507122
- Views 95
- Downloads 0
PLURIPOTENT STEM CELLS FOR VASCULAR DISEASE THERAPY
Vascular regeneration for ischemic disease represents an unmet clinical need. The ability to derive and expand homogenous populations of human vascular endothelial cells from either human embryonic stem (hES) cells or hu...
FEASIBILITY OF KONDAGOGU GUM AS A CARRIER FOR COLON TARGETED DRUG DELIVERY SYSTEM
The present study has been undertaken to develop colon targeted drug delivery system for metoprolol succinate using kondagogu gum as a polymer. Matrix tablets of metoprolol were prepared using PVP K-30 as binder and kond...
COMPARISON OF LIQUISOLID AND INCLUSION COMPLEXATION TECHNIQUE FOR DISSOLUTION RATE ENHANCEMENT OF VALSARTAN
The objective of this study is to screening of suitable non volatile liquid vehicle for the formulation of liquisolid tablets such as propylene glycol, polyethylene glycol 400 and tween 80 by using mathematical equations...
COMPARATIVE STUDY OF DIAGNOSTIC PROCEDURES IN SALMONELLA INFECTION, CAUSATIVE AGENT OF TYPHOID FEVER – AN OVERVIEW STUDY
Typhoid fever is a prolonged and debilitating fever which is of major public health concern in many countries.Laboratory diagnosis of typhoid fever requires isolation and identification of Salmonella enterica serotype Ty...
SIMULTANEOUS ESTIMATION AND VALIDATION OF PARACETAMOL, CHLORPHENIRAMINE MALEATE, PHENYLEPHRINE HYDROCHLORIDE, CAFFEINE AND ITS STRESS DEGRADATION STUDIES USING UV VISIBLE SPECTROSCOPY
A simple, precise, accurate and economic simultaneous UV spectrophotometric method has been developed for the estimation of Paracetamol, Chlorpheniramine Maleate, Phenylephrine Hydrochloride and Caffeine in tablet formul...