DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHODS FOR THE SIMULTANEOUS ESTIMATION OF MICONAZOLE NITRATE AND METRONIDAZOLE IN BULK POWDER FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 7
Abstract
Simple, precise, accurate and economical spectrophotometric methods have been developed for simultaneous determination of Miconazole nitrate (MIZ) and Metronidazole (MNZ) in their bulk powder form. Method (I) is simultaneous equation method and method (II) is absorbance ratio method. This is based on measurement of absorbance at two wavelengths 318.8 nm and 279 nm, λmax of Metronidazole and Miconazole nitrate in pH 4.0 phosphate buffers. Both the drugs obeyed Beer-Lambert’s law in the concentration range of 5-30 µg/mL for Metronidazole and 100-600 µg/mL for Miconazole nitrate. The high values of correlation coefficient (R2) indicated good linearity of calibration curve for both the drugs. The second method i.e. absorbance ratio method which involves the formation of Q-absorbance equation using the respective absorptivity values at 283 nm (isoabsorptive point) and 279 nm (λmax of miconazole nitrate); accuracy, precision and recovery studies of the developed methods have been carried out in order to confirm their accuracy.
Authors and Affiliations
Dhanashree Mundhey , Princy Morris , Govind Lohiya , Jasmine Avari
Keywords
Related Articles
- EP ID EP116270
- DOI 10.7897/2230-8407.0507122
- Views 117
- Downloads 0
STANDARDIZATION AND ANTIMICROBIAL ACTIVITY OF FICUS RELIGIOSA LINN. (FAMILY: MORACEAE)
Ficus religiosa Linn. (Moraceae) has been traditionally claimed to be useful in asthmatic conditions, antidiarrhoeal, antiviral and astringent. It also shows antivenom activity. It is used in the treatment of various dis...
A SIMPLE RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TENOFOVIR DISOPROXIL FUMARATE AND EMTRICITABINE IN TABLET DOSAGE FORM
A rapid, specific, sensitive and simple high performance liquid chromatography was developed for simultaneous estimation of Tenofovir Disoproxil Fumarate and Emtricitabine in tablet formulation. The separation was achie...
FINDING NEW INHIBITORS FOR EML4-ALK FUSION PROTEIN: A COMPUTATIONAL APPROACH
The fusion between echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK) has recently been identified in a subset of non-small cell lung cancers (NSCLC). PF-02341066 (crizotinib) is...
DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC AREA UNDER CURVE METHOD FOR SIMULTANEOUS ESTIMATION OF EPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM IN COMBINED CAPSULE DOSAGE FORM
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical area under curve method for the simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in combined capsule do...
PHYTO CHEMICAL SCREENING AND ANTI- OXIDANT ACTIVITY OF ETHANOLIC EXTRACT OF HELIOTROPIUM INDICUM
The aim of the present study was to investigate phyto chemical screening and anti-oxidant activity of the ethanol extract of the whole plant of Heliotropium indicum. The ethanolic extract of the whole plant of Heliotropi...