DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF BALOFLOXACIN IN BULK AND TABLET DOSAGE FORM
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: The objective of the method was to develop a simple, rapid, efficient, cost effective and reproducible, stability indicating (RP-HPLC) Reverse Phase High Performance Liquid Chromatography method for the estimation of Balofloxacin in bulk and tablet dosage form.Methods: The RP-HPLC analysis was carried out on an Enable C18 G column with a mobile phase of Potassium dihydrogen orthophosphate (pH adjusted to 2.5 with 0.1 M ortho phosphoric acid) and Acetonitrile in the ratio of 75:25% v/v. The analyte was detected at 293 nm using PDA detector. The method was validated in terms of linearity, accuracy, precision, LOD (Limit of Detection), LOQ (Limit of Quantification) and robustness as per ICH guidelines.Results: The method was found to be linear in the range of 10-120 µg/ml. Limit of detection and limit of quantitation was found to be 0.793 and 2.405 µg/ml respectively. Recovery was found to be in the range 100.268- 101.077 % and precision less than 2%. The developed method was successfully applied for the estimation of Balofloxacin in marketed tablet formulation (Baloforce TAB) and percentage assay was found to be 101.690 %. Forced degradation studies were performed under different conditions. The drug was degraded in acidic, basic, oxidative, UV light and sunlight conditions. The peaks of degraded products were well resolved from the actual drug. The results obtained prove that the developed method is a stability indicating method.Conclusion: The developed RP-HPLC method was simple, rapid, accurate, precise and stability indicating for the estimation of Balofloxacin in bulk and tablet dosage form.Â
Authors and Affiliations
Jasmine Dinyar Karanjia, Avani Chokshi
Keywords
Related Articles
- EP ID EP579727
- DOI -
- Views 81
- Downloads 0
IN-SILICO DESIGN, SYNTHESIS AND IN VITRO ANTITUBERCULAR ACTIVITY OF NOVEL 1, 2, 4 - TRIAZOLE DERIVATIVES
Objective: To design, synthesize and in vitro anti tubercular evaluation of some new 1,2,4–triazole derivatives.Methods: Novel 1,2,4–triazole derivatives were designed by using various softwares like ACD Lab Chemsket...
IN SILICO STUDIES ON NEW INDAZOLE DERIVATIVES AS GSK-3β INHIBITORS
Objective: In silico studies were conducted on newly proposed Indazole derivatives as GSK-3β inhibitors to select the best possible drug candidates based on drug properties and bioactivity score of the compounds.Methods...
STUDY TO EVALUATE THE ROLE OF SEROTONIN IN PARKINSONIAN SYMPTOMS
Objective: Study to evaluate the role of serotonin in Parkinsonism by using olanzapine (an atypical antipsychotic drug) on haloperidol-induced extrapyramidal symptoms (EPS) in Swiss albino mice.Methods: EPS like catatoni...
GANODERMA LUCIDUM-ORIENTAL MUSHROOM MEDIATED SYNTHESIS OF GOLD NANOPARTICLES CONJUGATED WITH DOXORUBICIN AND EVALUATION OF ITS ANTICANCER POTENTIAL ON HUMAN BREAST CANCER MCF-7/DOX CELLS
Objective: The present investigations are to mycosynthesis and characterization of gold nanoparticles conjugated with doxorubicin and evaluated anticancer activity.Methods: The characterization of the gold nanoparticles...
ANTIOXIDANT ACTIVITY AND ANTIBACTERIAL SCREENING OF TUBERS OF AMORPHOPHALLUS KONKANENSIS AND AMORPHOPHALLUS BULBIFER (ARACEAE)
Objective: Antioxidant activity (AOA) and antibacterial screening of tubers of unexplored Amorphophallus konkanensis Hett., Yadav & Patil (AKT) and Amorphophallus bulbifer (Roxb.) Bl. (ABT).Methods: Antioxidant activ...