DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHODFOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN AND OLMESARTAN IN PHARMACEUTICAL DOSAGE FORM

Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 6

Abstract

A simple, precise, rapid, specific and accurate stability indicating reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Atorvastatin (ATV) and Olmesartan (OLM) in pharmaceutical dosage form. Chromatographic separation was performed onAgilent eclipse XDB C8 (150x4.6mm, 5μ)column, with mobile phase comprising of mixture of buffer (pH3.25, adjusted with ortho phosphoric acid), and methanol in the ratio of 60:40v/v, at the flow rate 0.8 ml/min. The detection was carried out at 287 nm. The retention times of OLM and ATV were found to be 2.47 and 3.77 mins respectively with a run time of 6 mins, theoretical levels for OLM and ATV were 6248 and 4867 respectively, with a resolution of 7.57. As per ICH guidelines the method was validated for linearity, accuracy, precision, limit of detection and limit of quantitation, robustness and ruggedness. Linearity of OLM was found in the range of 120-360 μg/mL and that for ATV was found to be 60-180 μg/mL. The correlation coefficient for OLM and ATV were 1.000 and 0.999 respectively. The LOD values for OLM and ATV were 2.845 and 2.927 μg/mL respectively. The LOQ values for OLM and ATV were and 9.486 and 9.756 μg/mL respectively. This demonstrates that the developed method is simple, precise, rapid, selective, accurate and reproducible for simultaneous estimation of OLM and ATV tablet dosage form

Authors and Affiliations

Ranjeet Kumar V , Sai Malakondaiah D , Ajitha A, , Uma Maheshwara Rao V

Keywords

Related Articles

X-RAY FLUORESCENCE

X-ray fluorescence (XRF) spectrometry is an elemental analysis technique with broad application in science and industry. XRF is based on the principle that individual atoms, when excited by an external energy source, emi...

 EFFECT OF USING BLACK PEPPER AS NATURAL FEED ADDITIVE ON PERFORMANCE AND CARCASS QUALITY OF BROILER CHICKS

 Flowcytometry is a powerful technology mainly used for investigations in cell biology and isolation of individual cells.It is a laser based biophysical analytical technique used mainly for cell sorting, cell counti...

 DEVELOPMENT AND VALIDATION OF ASSAY METHOD FOR MELOXICAM TABLETS BY RP-HPLC

 ABSTRACT A simple, rapid, and accurate reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the determination of meloxicam .The separation was ca...

 COMPARATIVE STUDY OF LOCAL CONCENTRATE WITH IMPORT CONCENTRATE INCLUSION IN BROILER CHICKS DIETS

 A total of 120 broiler chicks, were used in this experiment to evaluate the effect of feeding local concentrate replaced the imported concentrate on the performance of broiler chicks. Three dietary treatments were...

COMPARATIVE ACCOUNT OF MEDICINAL PROPERTIES OF Andrographis paniculata AND Costus igneus

Both the plants, A. paniculata and C. igneus are easily grown in the kitchen garden of the back yard. Their phenolic contents are responsible for their antioxidant properties. The present paper has been undertaken to co...

Download PDF file
  • EP ID EP90325
  • DOI -
  • Views 189
  • Downloads 0

How To Cite

Ranjeet Kumar V, Sai Malakondaiah D, Ajitha A, , Uma Maheshwara Rao V (2014). DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHODFOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN AND OLMESARTAN IN PHARMACEUTICAL DOSAGE FORM. Int J of Pharm Res & Analy, 4(6), 382-387. https://europub.co.uk/articles/-A-90325