DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHODFOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN AND OLMESARTAN IN PHARMACEUTICAL DOSAGE FORM
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 6
Abstract
A simple, precise, rapid, specific and accurate stability indicating reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Atorvastatin (ATV) and Olmesartan (OLM) in pharmaceutical dosage form. Chromatographic separation was performed onAgilent eclipse XDB C8 (150x4.6mm, 5μ)column, with mobile phase comprising of mixture of buffer (pH3.25, adjusted with ortho phosphoric acid), and methanol in the ratio of 60:40v/v, at the flow rate 0.8 ml/min. The detection was carried out at 287 nm. The retention times of OLM and ATV were found to be 2.47 and 3.77 mins respectively with a run time of 6 mins, theoretical levels for OLM and ATV were 6248 and 4867 respectively, with a resolution of 7.57. As per ICH guidelines the method was validated for linearity, accuracy, precision, limit of detection and limit of quantitation, robustness and ruggedness. Linearity of OLM was found in the range of 120-360 μg/mL and that for ATV was found to be 60-180 μg/mL. The correlation coefficient for OLM and ATV were 1.000 and 0.999 respectively. The LOD values for OLM and ATV were 2.845 and 2.927 μg/mL respectively. The LOQ values for OLM and ATV were and 9.486 and 9.756 μg/mL respectively. This demonstrates that the developed method is simple, precise, rapid, selective, accurate and reproducible for simultaneous estimation of OLM and ATV tablet dosage form
Authors and Affiliations
Ranjeet Kumar V , Sai Malakondaiah D , Ajitha A, , Uma Maheshwara Rao V
EFFECT OF EXCIPIENTS AND PROCESS VARIABLES OVER GASTRO RETENTIVE ANTIHYPERTENSIVE DOSAGE FORM
The objective of this study was to formulate an oral floating tablet of Captopril using the hydrophilic polymer hydroxy propyl methyl cellulose (HPMC) and Carbopol 934P gas generating agent Calcium Carbonate and...
ISOLATION AND CHARACTERISATION OF MULTI DRUG RESISTANCE STRAINS FROM SKIN OF COWRANCH WORKERS
When drug resistance occurs, the drug or combination of drugs loses its ability to block the germ from reproducing. Over time, the treatment can stop working completely. This means the drugs become less effective....
DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF TRIFLUOPERAZINE IN BULK AND TABLET PHARMACEUTICAL FORMULATION
ABSTRACT The aim of present research work was to develop and validate UV spectroscopic method for estimation of Trifluoperazine in bulk and tablet pharmaceutical formulation. The method employed simple spectrosco...
A REVIEW ON COMBINATORIAL CHEMISTRY IN DRUG DISCOVERY
Combinatorial chemistry involves the rapid synthesis or the computer simulation of a large number of different but often structurally related molecules or materials. In a combinatorial synthesis, the number of comp...
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF NICOTINAMIDE AND CLINDAMYCINE IN BULK AND PHARMACEUTICAL FORMULATIONS
ABSTRACT The objective of the current study was to develop a simple, accurate, precise and rapid RP-HPLC method and subsequently validate as per ICH guidelines for the determination of Nicotinamide (NIC) and Clinda...