DEVELOPMENT OF A DENSITOMETRIC HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE ANALYSIS OF URSOLIC ACID IN THE LEAVES OF SPECIES OF GENUS TECOMA AND TABEBUIA OF BIGNONIACEAE FAMILY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 2
Abstract
Objective: A simple and sensitive high-performance thin–layer chromatographic (HPTLC) method was developed for the quantification of ursolic acid in the leaves of three species of genus Tecoma (Tecoma gaudichaudi DC, Tecoma capensis (Thunb.) Lindl, Tecoma stans (L.) Juss. ex Kunth) and genus Tabebuia (Tabebuia rosea Bertol) belong to family Bignoniaceae.Methods: Chromatography was performed on Silica gel 60 F254 precoated HPTLC Plates with optimised mobile phase pet ether: ethyl acetate: formic acid (7:3:0.5, v/v/v). The plate was derivatized with p-anisaldehyde reagent and scanned at 540 nm. The developed method was found to give a compact spot for ursolic acid at Rf value 0.43±0.01. The method was validated using International Council for Harmonization (ICH) guidelines, including linearity, precision, accuracy, and robustness.Results: Ursolic acid was found to be present in four species, i.e., Tecoma gaudichaudi DC (1.48%w/w), Tecoma capensis (Thunb.) Lindl. (0.79%w/w), Tecoma stans (L.) Juss. Ex Kunth (1.11%w/w), Tabebuia rosea (Bertol.) (1.13%w/w). A good linearity relationship was found to be (200-1400ng band−1) with a correlation coefficient (r2) value of 0.9946 with ursolic acid. Limit of detection and limit of quantification was considered to be 40.66, 123.21 ng per band respectively. The developed method was found to be accurate and precise with 1.32%, 1.19% (%RSD) for interday and intraday precision. The accuracy of the method was performed by recovery studies at three different concentration levels, and the average percentage recovery was found to be 98.05% for ursolic acid.Conclusion: The proposed method for the quantitation of ursolic acid was found to be reproducible and simple.
Authors and Affiliations
Kalyani A. Kedar, Sanjay R. Chaudhari, Avanapu S. Rao
Keywords
Related Articles
- EP ID EP575827
- DOI 10.22159/ijpps.2017v9i2.15802
- Views 94
- Downloads 0
FECUNDITY STUDIES OF ANDROGRAPHIS PANICULATA ON DROSOPHILA MELANOGASTER
Objective: Andrographis paniculata is widely used in Asia for various medicinal purposes. The plant has a major bioactive chemical constituent Andrographolide, which exhibits various essential pharmacological properties....
APPROACHES TO ACHIEVE AN ORAL CONTROLLED RELEASE DRUG DELIVERY SYSTEM USING POLYMERS: A RECENT REVIEW
The present article contains a brief review of various formulation approaches used in controlled release drug delivery systems, the role of polymers in the controlled delivery of many fast release drugs and the mechanism...
MODULATIONOF PUNGENCY AND MAJOR BIOACTIVE COMPOUNDS IN PEPPER DUE TO AGRO-CLIMATIC DISCREPANCY: A CASE STUDY WITH CAPSICUM CHINENSE BHUT JOLOKIA FRUIT
Objectives: The use of pepper fruit is renowned for its capsaicin content that has numerous beneficial effects in food and pharmaceutical industry. However pepper fruits exhibit wide variations in the accumulation of cap...
PECTINASE-MODIFIED RED GINSENG (GS-E3D) INHIBIT NF-ΚB TRANSLOCATION AND NITRIC OXIDE PRODUCTION IN LIPOPOLYSACCHARIDE-STIMULATED RAW 264.7 CELLS
Objective: Red ginseng has been used as traditional medicines and functional foods in the world, because of its health benefits. The aim of this study was to elucidate the anti-inflammatory effect and mechanism of pectin...
EVALUATION OF IMPACT OF PHARMACEUTICAL CARE ON IMPROVING KNOWLEDGE AND MEDICATION ADHERENCE IN CKD PATIENTS
Objectives: Evaluation of the impact of pharmaceutical care on improving knowledge and medication adherence in Chronic Kidney Disease (CKD) patients.Methods: Patient data were recorded on a data collection form after inf...