Development of a Novel, Rapid and Validated HPTLC Protocol for the Quantitative Estimation of Marrubiin from the Extract of Marrubium vulgare Linn
Journal Title: Journal of Advances in Medical and Pharmaceutical Sciences - Year 2016, Vol 10, Issue 3
Abstract
Aim: To develop a novel, simple, precise and rapid HPTLC protocol for the analysis of marrubiin (a furan labdane diterpene) in herbal extracts and formulations. Methodology: The marrubiin can be quantified by performing the HPTLC on silica gel F254 plates using toluene: ethyl acetate: acetic acid (5:4:1) as mobile phase. The developed method was validated as per ICH guidelines. Results: The protocol was found to be linear in the concentration range of 40-400 ng/spot and simultaneous comparison of Rf (0.47 ± 0.05) and overlapping UV spectra of samples confirm the specificity of the method. The limit of detection (LOD) and limit of quantification (LOQ) of biomarker were found to be 15 and 40 ng by the selected method. The low value of % relative standard deviation (less than 2) in peak area on analyzing the sample on same and different days ensures the precision of the developed method. Further the recovery of more than 95% of the marrubiin affirms the accuracy of developed analytical method. Conclusion: It can be concluded that the developed protocol could be beneficial for the qualitative and quantitative analysis of the marrubiin in herbal extracts and formulations.
Authors and Affiliations
A. Nanda, V. Mittal
Keywords
Related Articles
- EP ID EP328493
- DOI 10.9734/JAMPS/2016/29840
- Views 64
- Downloads 0
Safety Evaluation of Giant African Land Snails (Archachatina maginata) Haemolymph on Hematological and Biochemical Parameters of Albino Rats
The safety of Archachatina maginata haemolymph on biochemical and hematological indices of albino rats was investigated. Twelve white albino rats were grouped into 3 (A-C) of 4 animals each. Group A rats serve as control...
The Impact of Age in Prosthesis-patient Mismatch on Long-term Survival after Aortic Valve Replacement: in-vitro versus in-vivo Values
Aim: We studied the effect of age on survival in the setting of prosthesis-patient mismatch (PPM). Study Design: Retrospective single surgeon practice. Place and Duration of Study: Cardiothoracic department, Mater Dei Ho...
Potential 5-Fluorouracil Encapsulated mPEG-Chitosan Nanogels for Controlling Drug Release
Aims: The objective of this study was to prepare and characterize poly (ethylene glycol) methyl ether (mPEG)-conjugated chitosan (CS) nanogels, mPEG-CS, at different molar ratios for 5-Fluorouracil (5-FU) delivery (5-FU-...
Healthcare-seeking Practices for Common Childhood Illnesses in Northeastern Albania: A Community-based Household Survey
Introduction: Appropriate medical care-seeking could prevent a significant number of child deaths and complications due to ill health. Objectives: The purpose of our study was to assess care-seeking behaviour of mothers...
The Practice of Antibiotic Prophylaxis for Caesarean Delivery among Obtetricians and Trainee Obstetricians in Nigeria
Aims: Antibiotic prophylaxis is a recognized measure for preventing post-operative infection after Caesarean delivery and it is expedient that Obstetricians be aware and practice recommended regimen. This study aimed at...