DEVELOPMENT OF AN AMPEROMETRIC BIOSENSOR FOR THE ACETYLCHOLINE DETERMINATION IN BIOLOGICAL SAMPLES
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2018, Vol 15, Issue 2
Abstract
The work is devoted to the development of amperometric biosensor for the determination of acetylcholine in biological samples. In the work, two different variants of co-immobilization of choline oxidase and acetylcholinesterase were compared, and two-layer method of immobilization was selected (acetylcholinesterase-based membrane was deposited as the first layer, and choline oxidase-based membrane – as the second layer). Next, influence of the solution properties (buffer capacity, ionic strength, pH) on the biosensor analytical characteristics was studied. It was shown that the biosensor is characterized by good signal reproducibility and operational stability. Selectivity of the biosensor towards substances, which can be present in biological samples, was evaluated. Main analytical characteristics of the biosensor (sensitivity, linear range, limit of detection, noise, drift, reproducibility of responses) were determined. It was proven that the developed biosensor could be used for the determination of acetylcholine concentrations in biological samples.
Authors and Affiliations
D. V. Knyzhnykova, I. S. Kucherenko, О. О. Soldatkin, S. V. Dzyadevych, A. P. Soldatkin
Keywords
Related Articles
- EP ID EP399956
- DOI 10.18524/1815-7459.2018.2.136886
- Views 60
- Downloads 0
GAS SENSORS BASED ON ORGANIC-INORGANIC NANOCOMPOSITES
The use of nanocrystals with large specific surface in sensor structures provides their high sensitivity to molecules of adsorbed gases. The aim of the work was to create sensor elements based on composite films of poly-...
METHOD OF STENCILLING IN TECHNOLOGY OF DEVICES ON SURFACES ACOUSTIC WAVES
The method of acoustoabsorber deposing on acoustic ducts of devices on the surface acoustic waves, basing on stencilling which used at manufacturing of thick-film hybrid integrated circuits is described. The block diagr...
STUDY OF CHARACTERISTICS OF AMEROMETRIC NICKEL TRANSDUCERS USING POTENTIOSTAT ‘PALMSENS’ AND IT’S UKRAINIAN ANALOG
Characteristics of nickel amperometric transducers connected to experimental Ukrainian potentiostat and to commercial potentiostat PalmSens were studied in this work. During this, intrinsic characteristics of transducers...
SENSING INTERACTION DYNAMICS OF CHAOTIC SYSTEMS WITHIN A CHAOS THEORY AND MICROSYSTEM TECHNOLOGY GEOMATH WITH APPLICATION TO NEUROPHYSIOLOGICAL SYSTEMS
Paper is devoted to studying dynamics of the nonlinear neuro-physiological systems identifying the presence of chaotic elements. The mutual information approach, correlation integral analysis, false nearest neighbour alg...
ELECTROMAGNETIC WAVES RADIATOR WITH CONTROLLED CHARACTERISTICS
The problem of Н-sectorial horn-type radiator parameter improvement – the width of the main directional lobe (DL), side lobe level reduction, DL form monitoring with the help of a covering of an internal radiator cavity...