DEVELOPMENT OF AN AMPEROMETRIC BIOSENSOR FOR THE ACETYLCHOLINE DETERMINATION IN BIOLOGICAL SAMPLES
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2018, Vol 15, Issue 2
Abstract
The work is devoted to the development of amperometric biosensor for the determination of acetylcholine in biological samples. In the work, two different variants of co-immobilization of choline oxidase and acetylcholinesterase were compared, and two-layer method of immobilization was selected (acetylcholinesterase-based membrane was deposited as the first layer, and choline oxidase-based membrane – as the second layer). Next, influence of the solution properties (buffer capacity, ionic strength, pH) on the biosensor analytical characteristics was studied. It was shown that the biosensor is characterized by good signal reproducibility and operational stability. Selectivity of the biosensor towards substances, which can be present in biological samples, was evaluated. Main analytical characteristics of the biosensor (sensitivity, linear range, limit of detection, noise, drift, reproducibility of responses) were determined. It was proven that the developed biosensor could be used for the determination of acetylcholine concentrations in biological samples.
Authors and Affiliations
D. V. Knyzhnykova, I. S. Kucherenko, О. О. Soldatkin, S. V. Dzyadevych, A. P. Soldatkin
Keywords
Related Articles
- EP ID EP399956
- DOI 10.18524/1815-7459.2018.2.136886
- Views 51
- Downloads 0
STRUCTURAL-PHASE TRANSFORMATIONS AND ELECTROPHYSICAL PROPERTIES OF THE COMPOSITE MATERIALS BASED ON "SiO2-B2O3-Bi2O3-ZnO-BaO" SYSTEM
The processes of the glass forming and crystallization and also physical and chemical properties of "SiO2 - B2O3 - Bi2O3 - ZnO - BaO" system with the different concentration ratios of the initial components and doping im...
INFLUENCE OF IONS OF HEAVY METALS ON THE PHOTOLUMINESCENCE OF NANOCRYSTALS AGINS2 /ZNS
The aim of this work is to investigate the influence of ions of heavy metals Cu2+, Pb2+, Co2+, Ni2+, Cd2+, Ag+ , and ions, which are common components of natural and drinking water (Na+ , K+ , Ca2+, Mg2+), on the photolu...
DIRECTMEASURING PHASE METHOD OF SMALL DISTANCES MEASUREMENT BY THE OPTOELECTRONIC SENSOR IN DYNAMIC CONDITIONS
Statement of a task The work is devoted to development and researches of a method the small distances precision contactless measurement for creation of the optoelectronic sensor working in dynamic conditions. Technique...
GAS SENSORS BASED ON ORGANIC-INORGANIC NANOCOMPOSITES
The use of nanocrystals with large specific surface in sensor structures provides their high sensitivity to molecules of adsorbed gases. The aim of the work was to create sensor elements based on composite films of poly-...
OF IMITATION OF THE OPTICAL LOCATION DEVICE UNIFORM MOTION
The aim of the work is to develop a method of physico-mathematical modeling of opticalelectronic systems functioning conditions that solve the problem of reducing the time and cost of experimental studies. The main idea...