Development of cost-effective tetra-ARMS PCR for detection of FecB genotype in sheep
Journal Title: Animal Science Papers and Reports - Year 2014, Vol 32, Issue 3
Abstract
The FecB gene has been shown to be crucial in reproduction in many sheep breeds. It is a single nucleotide polymorphism (SNP) located in the bone morphogenetic protein receptor IB (BMPRIB) gene. The current methods for genotyping the FecB mutation are either slow and laborious or expensive. In this report, a single-step amplification approach suitable for FecB genotyping method is described. Multiplex PCR was performed with four primers on the basis of tetraprimer amplification refractory mutation system PCR (tetra-primer ARMS PCR), then three FecB genotypes can be detected after electrophoresis. Genotyping results of the proposed multiplex PCR occurred to be in complete accordance with forced PCR-RFLP of all samples. It is a rapid and simple method for detection of FecB in a larger number of samples, and is suitable for other SNPs detection with specific primers even in most “low-tech” laboratories.
Authors and Affiliations
Feng Guan, Guoqing Shi, Pengcheng Wan, Rong Dai, Hong Tang, Haixia Wang, Yuanyuan Luo
Keywords
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