Development of Monoclonal Antibody Anti-African Bitis arietans Snake Toxin Phospholipase A2

Journal Title: Journal of Toxins - Year 2017, Vol 4, Issue 1

Abstract

This work is part of long-term research to improve the quality of antivenom antibodies based on cumulative information gathered from established knowledge of the immune response cellular and molecular mechanisms as well as from molecular animal venom toxins. There are two main objectives: first, the introduction of punctual modifications to antitoxin antibody (Ab) production for immediate use; second, the use of Ab hyper variable regions to model scFvs that have high toxin neutralizing potency. The first objective has been successfully accomplished in recent years. Polyclonal Abs (pAbs) that has high antigen (Ag) potency recognition has been developed. For the second objective, the use of purified-characterized toxins as antigens to produce monoclonal Abs (mAbs) is ongoing. This manuscript contains data indicating that purified and enzymatically characterized PLA2 from Bitis arietans venom induces IgA mAbs that recognize epitopes and neutralize toxic domains with desirable potency. The obtained mAb will serve as a hypervariable sequence source for single chain fragments variable (scFvs). The present study highlights the use of purified venom toxins as substitutes for complete venoms in immunization procedures.

Authors and Affiliations

Wilmar Dias da Silva

Keywords

Related Articles

Determination of Iron, Copper, Zinc and Manganese in Sausage, Poultry-Rabbit Meat, Viscera and Red Meats Consumed by the Population in The Canary Islands, Spain

The aim of this work was to determine the levels of Fe, Cu, Zn, and Mn in samples of sausage, bird poultry-rabbit meat, viscera and red meats, collected in the Canary Islands (Spain). Fe, Cu, Zn, and Mn were analysed by...

Development of a New Method for the Determination of Manganese, Cadmium, Mercury and Lead in Whole Blood and Amniotic Fluid by Inductively Coupled Plasma Mass Spectrometry

Environmental exposure to metals among women, revealed their adverse effects on pregnancy. During this stage, blood levels of some metals increase so research on heavy metals transference from the mother’s blood to the d...

Do Diesel Exhaust Exposure andSmoking Affect Health of Employees at A Logistic Company in Western Australia?

Objective: This study aimed to introduce a semi-quantitative approach and assess historical occupational diesel exhaust exposure, tobacco smoking and related health effects in workers in Western Australia. Materials and...

Mutagenicity Analysis of C8-Phenoxy-Guanine in the NarI Recognition DNA Sequence

Phenoxyl radicals can covalently attach to the C8-site of 2′-deoxyguanosine (dG) to generate oxygen-linked biaryl ether C8-dG adducts. To determine the mutagenicity of an O-linked C8- dG adduct, C8-phenoxy-dG (PhOdG) was...

Chemoprevention of Silymarin and Vitamin C on Isoniazid-Induced Hepatotoxicity in Experimental Rat Model

Isoniazid, anti-mycobacterial agent employed clinically in the treatment of bacterial infections (tuberculosis), is known to cause a number of biochemical dysfunctions and suspected to induce hepatic damage to animals an...

Download PDF file
  • EP ID EP246749
  • DOI 10.13188/2328-1723.1000020
  • Views 120
  • Downloads 0

How To Cite

Wilmar Dias da Silva (2017). Development of Monoclonal Antibody Anti-African Bitis arietans Snake Toxin Phospholipase A2. Journal of Toxins, 4(1), 1-7. https://europub.co.uk/articles/-A-246749