Development of Monoclonal Antibody Anti-African Bitis arietans Snake Toxin Phospholipase A2
Journal Title: Journal of Toxins - Year 2017, Vol 4, Issue 1
Abstract
This work is part of long-term research to improve the quality of antivenom antibodies based on cumulative information gathered from established knowledge of the immune response cellular and molecular mechanisms as well as from molecular animal venom toxins. There are two main objectives: first, the introduction of punctual modifications to antitoxin antibody (Ab) production for immediate use; second, the use of Ab hyper variable regions to model scFvs that have high toxin neutralizing potency. The first objective has been successfully accomplished in recent years. Polyclonal Abs (pAbs) that has high antigen (Ag) potency recognition has been developed. For the second objective, the use of purified-characterized toxins as antigens to produce monoclonal Abs (mAbs) is ongoing. This manuscript contains data indicating that purified and enzymatically characterized PLA2 from Bitis arietans venom induces IgA mAbs that recognize epitopes and neutralize toxic domains with desirable potency. The obtained mAb will serve as a hypervariable sequence source for single chain fragments variable (scFvs). The present study highlights the use of purified venom toxins as substitutes for complete venoms in immunization procedures.
Authors and Affiliations
Wilmar Dias da Silva
Keywords
Related Articles
- EP ID EP246749
- DOI 10.13188/2328-1723.1000020
- Views 118
- Downloads 0
Estrous Cycle Cessation Caused via Activating the Apoptotic Pathway by NNSS2 Isolated from Naja naja Shedded Skin
The snake shed skin has long been used in the Chinese and Indian traditional, folk medicine for various medicinal purposes. Previously it was reported that N. naja shedded skin caused temporary cessation of the estrous c...
What Should We Do After Acute Organophosphorus Pesticide Poisoning During Pregnancy?
A 25-year-old married primigravida was admitted to Pingjin Hospital after ingestion of dichlorvos, with suicidal intent. The initial serum dichlorvos level was 1.7 umol/L, with a reduction in cholinesterase activity of 2...
Development of a New Method for the Determination of Manganese, Cadmium, Mercury and Lead in Whole Blood and Amniotic Fluid by Inductively Coupled Plasma Mass Spectrometry
Environmental exposure to metals among women, revealed their adverse effects on pregnancy. During this stage, blood levels of some metals increase so research on heavy metals transference from the mother’s blood to the d...
Chemoprevention of Silymarin and Vitamin C on Isoniazid-Induced Hepatotoxicity in Experimental Rat Model
Isoniazid, anti-mycobacterial agent employed clinically in the treatment of bacterial infections (tuberculosis), is known to cause a number of biochemical dysfunctions and suspected to induce hepatic damage to animals an...
In vitro and In vivo Anti-Arthritic and Anti-Inflammatory Activity of Bungarus Fasciatus Venom
In the Indian traditional medicinal system Ayurveda and Unani, the use of snake venom has been mentioned to treat various diseases including arthritis. This prompted us to investigate the anti-arthritic andanti-inflammat...