Abstract

The generation of doubled haploids in anther culture aims to accelerate the acquisition of pure lines within a short span of time. Selection of the desired traits can be done directly from anther culture resulted progeny at early generations. However, there is a possibility of segregation in doubled haploids lines in the future for which this experiment was taken up to determine the agronomic traits, uniformity and stability of the DH lines derived from a popular rice hybrid, BS6444G. The first experiment used 200 DH lines of the first generation of anther culture results (A1) following completely randomized design in two years showed each line has uniform agronomic traits; variation was observed in all 200 DHs. However, based on the better performance, 13 DH lines were selected for further evaluation along with the parent hybrid and two better checks in split plot for four seasons. The results showed that the progenies of individuals of 13 DH lines derived from the second to fifth generations showed no difference between generations for each agronomic trait of the same lines which were stable from generation to generation. Simultaneously, SSR markers also confirmed the stability of all the 13 selected DH lines showing homozygosity in all individual DH populations. Therefore, the selected DH lines could be efficiently used for the successful products in future. The 6 selected DH lines could reach the heterotic level over-yielding (5.4%- 41.9%) than the hybrid and better checks.

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