Development of random amplified polymorphic DNA markers for authentication of Croton tiglium Linn.

Journal Title: UNKNOWN - Year 2017, Vol 6, Issue 3

Abstract

Background: Croton tiglium Linn., commonly known as Jayapala, in Ayurvedic pharmacopoeia, is wellknown for its purgative action. In the herbal raw drug market, seeds of Baliospermum montanum Blume., Ricinus communis Linn. and Croton roxburghii Wall. etc. are sold in the name of Jayapla seeds due to their morphological similarities. Hence their identification through molecular characters is need of the hour. Aim: Present study aims with the molecular characterization of young leaves of C. tiglium Linn. Materials & Methods: Fresh young leaves of C. tiglium Linn. were collected from its natural habitat Udupi, Karnnataka, during January 2017. It was used for molecular characterization and DNA fingerprints, by standard and most convenient Random Amplified Polymorphic DNA (RAPD) markers at Food testing laboratory, Junagadh Agriculture University, Gujarat, India. Results: All the primers gave good band patterns. Primer 5, 6, 8, 11 and 13 showed more number of light and bright bands matching characters with plant. Conclusion: Observed RAPD marker can be used to differentiate genuine as well as adulterated samples. The results may be used for the further research purposes and also required DNA Barcoding studies for further authentication.

Authors and Affiliations

Shweta Vekariya, Krushnkumar Taviad, Acharya RN, Harisha CN

Keywords

Related Articles

Ingredient identification and phytochemical evaluation of Shalmali kantaka cream

Background: In any system of medicine the drug identification is the most essential task prior to any treatment plan. In Ayurved it has more significance as there are various plants available with similar characteristics...

Chemical constituents and pharmacological action of Ocimum sanctum (Indian holy basil-Tulsi)

The plant of Oscimum sanctum commonly known as “Tulsi”, belong to Lamiaceae family. It is an erect, much branched, fragrant and erected plant attaining a height of about 20-50 cm. Different parts of the plant are used in...

Bioefficacy of Selected Plant Extracts against Sitophilus zeamais on Post-Harvest Management of Zea mays

Maize (Zea mays L.) is the most important cereal crop in Kenya with 90% of the total population relying on it as the main staple food. Post-harvest loss in maize is caused by insect pests such Sitophilus zeamais and Larg...

Preclinical aphrodisiac investigation of ethanol extract of Flueggea leucopyrus Willd. leaves

Sexual dysfunction is a widely found disorder worldwide now a day because of busy lifestyle and stress. Management of disease is somewhat difficult because of cost and shortage of effective treatment. In the present pape...

Acute toxicity of red fruits (Pandanus conoideus Lamk) oil and the hepatic enzyme level in rat

Red fruit (Pandanus conoideus Lamk) empirically has been used Papuapeople , Indonesia as a natural medicine to treat a variety of diseases including cancer, HIV / AIDS, herpes and diabetes. The information about the toxi...

Download PDF file
  • EP ID EP451505
  • DOI -
  • Views 67
  • Downloads 0

How To Cite

Shweta Vekariya, Krushnkumar Taviad, Acharya RN, Harisha CN (2017). Development of random amplified polymorphic DNA markers for authentication of Croton tiglium Linn.. UNKNOWN, 6(3), 164-166. https://europub.co.uk/articles/-A-451505