DEVELOPMENT OF REVERSED-PHASE HPLC METHOD FOR ISOLATION AND QUANTIFICATION OF RUTIN AND QUERCETIN FROM FLOWER BUDS OF SOPHORA JAPONICA L.
Journal Title: International scientific journal Science and Innovation - Year 2024, Vol 3, Issue 10
Abstract
In the article, the results of the extraction of rutin and quercetin, which formed the basis of the flower buds of Sophora Japonica L. grown in the territory of Syrdarya region, and the quantitative analysis of the extracted polyphenolic compounds by the method of high-performance liquid chromatography (HPLC) were described. A linear relationship with the coefficient of determination (R2) was observed for rutin and quercetin in the range of 25-500 μg. This relationship was found to be y=13970x+13878 and y=46919x+287504 at retention times of 2.407 and 6.452 minutes, respectively. The LODs for rutin and quercetin were found to be 12.74 and 13.48 µg/ml, respectively. The LOQs were found to be 38.59 and 40.86 µg/ml, respectively. Rutin was the most flavonoid analyzed in Sophora Japonica L. and when isolated by the extraction method, the purity rate for HPLC was found to be 94.483%. Quercetin, obtained by acid hydrolysis of rutin, was found to have an HPLC purity of 94.238%.
Authors and Affiliations
Mengliev M. U. , Abdurakhmanova U. K.
Keywords
Related Articles
- EP ID EP750140
- DOI 10.5281/zenodo.14015792
- Views 52
- Downloads 0
STRATEGIES FOR DEVELOPING THE EFFECTIVE MANAGEMENT SYSTEM OF SPORTS FEDERATIONS AND ASSOCIATIONS
In addition to being a member of prestigious international organizations, the Republic of Uzbekistan has become an equal member of a number of international organizations in order to develop the field of physical educati...
IMPROVING THE USE OF INTERACTIVE METHODS IN TEACHING THE RUSSIAN LANGUAGE TO VETERINARY STUDENTS
Focusing on interactive methods in foreign language teaching increases the effectiveness of teaching and broadens the student’s horizons along with language learning. This article provides students majoring in veterinary...
THE STRUCTURE AND FUNCTIONAL CHARACTERISTICS OF HUMAN NERVOUS TISSUE
The human nervous system is a highly specialized and intricate network that coordinates sensory input, motor output, and cognitive functions. This paper investigates the histological structure and functional roles of ner...
DEVELOPING PROFESSIONAL COMMUNICATION SKILLS IN ENGLISH OF AGRICULTURAL EDUCATION STUDENTS
This article discusses the methodology of teaching professional communication using technology. It describes the basic principles and approaches to learning communication skills, including the use of various technologies...
PREGNANCY WITH CONGENITAL HEART DISEASE
Congenital heart disease (CHD) is a defect in the structure of the heart and large vessels diagnosed from the moment of birth. The frequency of occurrence varies widely and ranges from 2.4 to 14.15%. Every year, more tha...