Dextran-Conjugated Anti-Immunoglobulin Antibodies: A Powerful Tool for Studying B Cell Receptor-Mediated Signaling
Journal Title: International Journal of Immunology and Immunotherapy - Year 2016, Vol 3, Issue 2
Abstract
Dextran-conjugated anti-immunoglobulin (Ig) antibodies (anti-Ig-dex) consist of a high molecular weight dextran (~1-2 × 106 MW) to which multiple monoclonal anti-IgM or anti-IgD antibodies are covalently linked. These soluble conjugates have been used in a process of discovery, in extensive studies of in vitro polyclonal murine and human B cell activation, proliferation, Ig secretion and class switching, through signaling via multivalent cross-linking of B cell receptors (BCR) on the B cell surface, in the presence of a multiplicity of added stimuli. The cross-linking of the BCR in a multivalent fashion represents a more physiologic means, relative to using unconjugated bivalent anti-Ig, for studying the functional consequences of B cell encounter with specific antigens in vivo. This reflects either the repeating epitope nature of polysaccharides, or the display of a given intact protein in multiple copies on the surface of follicular dendritic cells, macrophages and dendritic cells where they are encountered by B cells.
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