DIAGNOSIS OF CANINE DEMODICOSIS BY INDIRECT- ELISA IN HYDERABAD OF TELANGANA STATE
Journal Title: International Journal of Agriculture Sciences - Year 2017, Vol 9, Issue 2
Abstract
Canine demodicosis is an inflammatory, non pruritic and non contagious condition is characterized by the presence of numerous mites on the skin. Skin scraping, trichoscopy and skin biopsy are the common diagnostic methods. The existence of subclinical and asymptomatic infection in the skin makes it difficult for veterinarians to diagnose the infection. Hence the present study was aimed to develop a laboratory standardized Indirect ELISA for diagnosis and seroprevalence of canine demodicosis in and around Hyderabad. Demodex canis antigen concentration was optimized at 0.5 µg per well of ELISA nunc plate against 1:50 anti- Demodex positive sera and 1:5000 dilution of Rabbit anti-dog IgG HRPO conjugate. Out of 88 samples collected from animal birth control centers 17 were positive (19.31 %) and out of 72 samples from pet clinics 22 were positive (30.55 %) with Indirect ELISA. The overall sensitivity of test was 70.58% and specificity was 76.92%.
Authors and Affiliations
G. S. SREENIVASAMURTHY
Keywords
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