DIAGNOSIS OF MDR TUBERCULOSIS AT A TERTIARY CARE CENTRE WITH CBNAAT TECHNOLOGY- A TWO YEAR STUDY
Journal Title: Journal of Evidence Based Medicine and Healthcare - Year 2018, Vol 5, Issue 53
Abstract
BACKGROUND Tuberculosis is a chronic multi system granulomatous disease with a predilection for the respiratory system. Traditional diagnostic protocols include history, chest x-ray and sputum collection and staining for acid fast bacilli along with culture in conventional media. These processes are time consuming and labour intensive. WHO recommends the use of Cartridge Based Nucleic Acid Amplification Testing (CBNAAT) using the Gene Xpert MTB/RIF apparatus which is a comparatively rapid PCR based test. MATERIALS AND METHODS In the present study, sputum and tissue samples from 1299 patients were taken and smear preparations were stained for acid fast bacilli. Subsequently, the samples were subjected to CBNAAT testing for both the presence of mycobacterium tuberculosis as well as rifampicin resistance, which is a marker for INH resistance as well. Rifampicin resistant tuberculosis is thus labelled as multi drug resistant tuberculosis (MDR TB). RESULTS In the results of the study, mycobacterium tuberculosis (MTB) was found in 1275 cases, Rifampicin sensitivity was found in 1095 cases, rifampicin resistance in 176 cases and indeterminate results in 4 cases. In 122 cases of the present study, CBNAAT has detected mycobacterium tuberculosis (MTB) where the cases were smear negative. On the other hand, 109 smear positive cases have been declared MTB negative by CBNAAT testing. 26 cases showed error, invalid test and no results. In 4 cases though MTB was detected, rifampicin resistance testing showed indeterminate results. CONCLUSION CBNAAT technology may be used as the standard diagnostic tool for tuberculosis. However for disputed cases, referral systems must be in place for mycobacterial culture and drug testing at reference laboratories.
Authors and Affiliations
Puranjay Saha, Royani Saha
Keywords
Related Articles
- EP ID EP610089
- DOI 10.18410/jebmh/2018/743
- Views 100
- Downloads 0
DETERMINANTS OF PRIMARY CAESAREAN DELIVERY AND ITS OUTCOME IN SOUTH KERALA
BACKGROUND Caesarean Delivery is one of the most common surgical intervention in modern obstetrics. It is certainly one of the oldest operations in surgery, with its origin lost in antiquity and ancient mythology. Caesar...
RESPIRATORY PROBLEMS IN NEWBORNS ASSOCIATED WITH MECONIUM-STAINED AMNIOTIC FLUID
BACKGROUND Perinatal morbidity and mortality resulting from aspiration of Meconium-Stained Amniotic Fluid (MSAF) is due to the respiratory problems ranging from mild respiratory distress to meconium aspiration syndrome a...
COMPARATIVE STUDY OF TOPICAL MOMETASONE FUROATE 0.1%, TOPICAL 0.03% TACROLIMUS, TOPICAL BASIC FIBROBLAST GROWTH FACTOR (bFGF) IN CHILDHOOD VITILIGO
BACKGROUND Vitiligo, the commonest of all pigmentary disorders, is an idiopathic, acquired cutaneous achromia, characterised by circumscribed, chalky white macules. It may also involve the pigment epithelium of the eyes,...
THE PATTERN OF SEXUAL MATURITY RATE OF GIRLS AGED BETWEEN 8-17 YEARS IN AND AROUND TIRUPATI
The pattern of development from the childhood to womanhood is of an orderly nature and is to some extent influenced by many external and internal influences.1 The first of the development order is the Thelarche (a) the d...
GLAUCOMA AWARENESS AMONG MEDICAL STUDENTS OF A MEDICAL COLLEGE IN NORTH INDIA
BACKGROUND In many parts of India Glaucoma patients present for Ophthalmology check-up when their disease is in advanced stage and at that point of time majority of their vision is already lost. There are many studies ad...