Diagnostic performance of automated Analyzers versus manual method for WBCs measurement among Population of Wad Madani, Gezira State, Sudan
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 3
Abstract
Abstract: The automated hematology analyzer with complete blood count (CBC) results has replaced the traditional manual or individual assay methods for hematological parameters and the eye count leukocyte differential as the initial screening and detection system for hematological abnormalities in modern hospitals and clinics. Automated method for the estimation of complete blood count is commonly used in routine practice laboratory but many other labs still work on manual procedure for the abnormal automated results as well as health care workers in laboratory can be optimized by doing test on manual microscopic procedure as validation technique for automated method. This study carried out to compare the WBCs by different automated analyzers and manual method. Study carried out in Wad Madani city capital of Gezira State, central Sudan during the period of July to August 2016. The study included 75 healthy individuals selected randomly, 31 of them were males and 44 were females, their age ranges from 20 to 50 years who came for medical examination. Three mls of venous blood were collected from participants in EDTA anticoagulant. The samples were analyzed using haemocytometer, Sysmex XP-300 and Mindray BC-6800.The results showed that repeatability of white blood cells by chamber, mindray and sysmex were (5.1707, 5.0293, 4.9560), (5.8680, 5.8573, 5.8507) and (6.0093, 5.9800, 5.9627) ×10³µl respectively, there was no significant different between the repeated result for manual and mindray but there was significant different by sysmexP.value (0.04). And when we compared the means+ SD of white blood cells by manual, mindray and sysmex were (5.0520±1.6460), (5.8587±1.7355) and (5.9840±1.7130)× 10³µl respectively, there was highly significant different by all three method P.value (0.001).Our study concluded that, there was no significant difference between repeatability of WBCs by manual and Mindray, significant difference between repeatability of WBCs by Sysmex and application of automated method is easier when there is load of work which takes less time. Keywords:White blood cells, automated hematology Analyzers,Manual Method
Authors and Affiliations
Tayseer Ibrahim Elsidigge, AlbadawiAbdelbagi Talha, Mohammed Siddig Younis
Keywords
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