Diagnostic Utility of Plasma Chitotriosidase, Total Acid Phosphatase Activity, Ferritin and Globulin Levels in Screening for Gaucher Disease
Journal Title: International Journal of Biochemistry Research & Review - Year 2017, Vol 16, Issue 4
Abstract
Aims: Gaucher disease "GD" is one of the most common glycolipid storage disorders. Several circulating molecules thought to be secreted by Gaucher cells have been studied as biological markers of disease. We investigate and compare the validity of using plasma chitotriosidase "ChT", total acid phosphatase activity, ferritin, and globulin in predicting GD in pediatric patients. Methodology: A cross sectional hospital based study, carried out on 43 pediatric patients (23 GD patients and 20 patients with suspected storage disorder). Complete blood counts, globulin assays, calculated A/G ratio, β-glucocerebrosidase activity assays, plasma protein electrophoresis, ELISA assays of plasma ChT and ferritin, colorimetric assay of plasma total acid phosphatase activity and GBA gene mutation analysis, were done for the included patients. Results: Significantly higher plasma ChT levels and increased total acid phosphatase activity were observed in GD patients versus those suspected to have storage disorders (P-value <0.05). High plasma ChT and ferritin have higher sensitivity, while high plasma total acid phosphatase activity, and globulin and low A/G ratio have higher specificity in diagnosing GD. Conclusions: Plasma ChT and total acid phosphatase activity, both together are more valid than ferritin and globulin in screening for GD.
Authors and Affiliations
Mohammed H. Hassan, Ayat A. Sayed, Ahmed El-Abd Ahmed, Tahia H. Saleem
Keywords
Related Articles
- EP ID EP317132
- DOI 10.9734/IJBCRR/2017/32782
- Views 135
- Downloads 0
In vitro Antisalmonellal and Antioxidant Activities of Extracts and Fractions of Curcuma longa L. Rhizomes (Zingiberaceae)
Typhoid fever is still a major public health problem in developing countries, where it remains endemic. With a view to searching for new antityphoid substances, Curcuma longa rhizome extracts and fractions were assessed...
Biochemical Impacts of Radiation Processing on Grapes and Artichoke Agro Industrial Wastes
Food irradiation is a method of preservation used to extend the shelf life of food products fresh and/or dried, destroy the contaminating harmful pathogens and modify the activity of bioactive compounds present in food m...
A Chito-specific, Adenine Binding Agglutinin from Benincasa hispida Shows High Structural and Functional Stability
Aims: To purify and characterize a novel chito-specific lectin from Benincasa hispida fruit. Study Design: Protein purification and characterization using biochemical and biophysical techniques under various denaturing c...
Diabetes in Pregnancy Study Group in India (DIPSI) – A Novel Criterion to Diagnose GDM
Background: Gestational diabetes mellitus (GDM) is defined as glucose intolerance recognised first only during pregnancy. Women with GDM are more prone to future diabetes and other maternal and fetal complications. Most...
Protective and Curative Antiobesity Potential of Lemon Peel Extract in Rats Fed on High Fat Diet: Mechanism of Action
Aim: This study was to investigate the effect of dietary lemon peel extract (LPE) on high fat diet induced obesity in rats. Some of the LPE mechanism of action was also elucidated. Fifty adult rats were divided into five...