DIFFERENT METHODS OF DERMATOPHAGOIDES FARINAE SENSITIZATION DETERMINATION IN PATIENTS WITH RESPIRATORY ALLERGY
Journal Title: Астма и Аллергия - Year 2019, Vol 1, Issue 2
Abstract
Objective was to study and compare the parameters of the specificity and sensitivity of skin testing and serologic determination of specific Dermatophagoides farinae IgE. Materials and methods . 88 patients with allergic rhinitis and/or atopic bronchial asthma were examined by three different methods of specific allergic diagnosis (in vivo and in vitro) in accordance with the guidelines of the ethics committee of the National Pirogov Memorial Medical University, all were beyond the acute period. The inclusion criteria were allergic rhinitis and/or atopic bronchial asthma diagnosis (both intermittent and persistent) with proven sensitivity to domestic allergens. Skin prick test was carried out by classical testing procedure in accordance with regulatory documents with commercial extracts of allergens. Western blot testing for specific IgE levels was performed using RIDA qLine test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun). The sIgE concentration was converted to a nominal scale (grades) according to the following rules: < 0.35 IU mL-1-level 0 (negative), (0.36–0.69) IU mL-1-level 1 (boundary levels ), (0.7–3.49) IU mL-1-level 2 (slightly elevated), (3.50–17.4) IU mL-1-level 3 (moderately elevated), (17.5–49.9) IU mL-1-level 4 (high levels), (50–100) IU mL-1-level 5 (very high levels) and > 100 IU mL-1-level 6 (extremely high levels). Results and discussion. The results of two systems for determining specific IgE to D. Farinae by methods Rida AllergyScreen and Euroline are consistent with each other, but there is a systematic difference in indicators (-0.71 kU/l). There is good agreement between the data of skin testing with allergens D. Farinae and the detection of specific IgE by the method of Rida AllergyScreen, but there is a satisfactory agreement between the results of skin testing with D. Farinae allergens and the detection of specific IgE by the Euroline method.
Authors and Affiliations
A. Ye. Bogomolov
Keywords
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- EP ID EP647843
- DOI 10.31655/2307-3373-2019-2-24-28
- Views 98
- Downloads 0
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