Differential expression of Isoesterases in leaves and roots of Vigna unguiculata L. in response to saline stress
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2012, Vol 8, Issue 2
Abstract
The present study was intended to reveal the differential isoesterase expression in leaves and roots of Vigna unguiculata L. in response to saline stress. Influence of NaCl (0, 3, 5, 7 and10 %) on seedlings of Vigna unguiculata L. was analyzed. The seedlings were grown in the centre for a period of 30 days. The randomly collected whole plants were used as a source for isoesterase isolation. The seedlings showed the maximum tolerance up to 10% of NaCl. The Poly acrylamide gel electrophoresis was performed by Anbalagan method. The staining and fixation of the enzyme was performed by the Sadasivam and Manickam method. Seedlings treated with various concentrations of NaCl showed different banding profile based on the concentration (0-10%) and duration (5-30 days) of salt treatment as follows: 32 bands with five active regions, 56 bands with eight active regions, 102 bands with eight active regions, 47 bands with seven active regions, 64 bands with nine active regions and 84 bands with nine active regions on 5th, 10th, 15th, 20th, 25th and 30th day respectively. The changing pattern of isozymes during development may be interpreted as evidence for differential timing of gene expression correlated with the physiological stress. The results of the present study concluded that the isoesterase patterns could serve as a useful biochemical marker of salinity.
Authors and Affiliations
M. Johnson
Keywords
Related Articles
- EP ID EP119637
- DOI -
- Views 115
- Downloads 0
Cold Storage of Plant Tissue Cultures – Effect of Sucrose
Explants of normal (non-transformed) carrot ([i]Daucus carota[/i] L.) tissue, scorzonera crown gall ([i]Scorzonera hispanica[/i] L.) and carrot tissues transformed with [i]Agrobacterium tumefaciens[/i] (all cultured in v...
Gallic Acid Protects Against Immobilization Stress-Induced Changes In Wistar Rats
[b]Background:[/b] Stress triggers a wide range of body changes. Herbal medicines are rich in non specific antistress agents. [b]Purpose:[/b] The present study was carried out to evaluate the antistress effect of gallic...
Effects of Water Stress on Rice Production: Bioavailability of Potassium in Soil
Water demand in agriculture, municipal, and industrial purposes is increasing rapidly which will pressure on future demand in agriculture. To justify less water use in rice production, we produced rice under different wa...
The Responses of Ascorbate - Glutathione Cycle Enzymes in Seedlings of Pancratium maritimum L. under Drought Treatments
In this study, physiological and biochemical responses of (Pancratium maritimum L.), desert plant which is very widespread on coastal sand dunes to drought were determined. Therefore 28 days (d) old plants were drought s...
THE EXPERIENCE OF THE TRANSFORMATION OF SOME CULTIVATED PLANTS WITH THE GENE UGT ENCODING THE SYNTHESIS OF UDPG-TRANSFERASE IN ORDER TO CHANGE THE HORMONAL STATUS