Differential expression of MU [μ] Opioid receptors and N-type calcium channels in the amygdala of morphine tolerant rat: An autoradiographic & immunohistochemistry study
Journal Title: Indian Journal of Clinical Anatomy and Physiology - Year 2016, Vol 3, Issue 4
Abstract
Introduction: Recent studies have shown that amygdala plays a pivotal role in the emotional processing of pain. Opioids like morphine relieve pain by suppressing both the sensory and emotional components of pain. On chronic morphine administration, tolerance develops decreasing its analgesic effect. The underlying changes in the amygdala during morphine tolerance are not definitely known. Mu opioid receptors are binding sites for morphine while N-type voltage-gated calcium channels (VGCCs) directly control the release of neurotransmitters from presynaptic nerve endings. Aims & Objectives: The present study was designed to investigate the status of mu receptors and N-type VGCCs in the amygdala of morphine tolerant rat by autoradiography and immunohistochemistry respectively. Material & Methods: Wistar rats were made morphine tolerant by repeated administration of morphine for 5 days. Later, the rats were euthanized and cryostat sections of the brain were cut at the region of the amygdala. For autoradiographic localization, the sections were incubated with [3H] labeled DAMGO. For immunohistochemistry, the sections were exposed to primary antibody against N-type VGCCs. Results: Using image analysis software, a significant down-regulation of mu receptors and an up-regulation of N-VGCCs were observed after tolerance. This differential change in expression would indicate a less effective antinociceptive action of morphine through / though higher neurotransmitter release after tolerance. Conclusion: In conclusion, the study suggests that morphine tolerance could be associated with altered functional state of the amygdala.
Authors and Affiliations
Satya Narayan Shukla, Sarabpreet Singh, SB Ray
Keywords
Related Articles
- EP ID EP242922
- DOI -
- Views 67
- Downloads 0
A cadaveric study of coronary arteriesin Rayalaseema population of Andhra Pradesh
Introduction Majority of the sudden cardiac deaths were due to anomalous origin and distribution of the coronary arteriesAim To study the arterial vascular pattern of the human heart by using dissection method in Rayalas...
A cross sectional study of the perceptions of first year medical students regarding Post graduate preferences
Introduction: Growing appreciation of issues related to post graduate preferences among undergraduate medical students in recent years assumes great importance in health care delivery of the country. This study was carri...
Variable placental attachment of umbilical cords and its effects on placental outcomes
Introduction: Growth of the fetus is dependent on the development and function of the placenta. Therefore, any pathological condition which affects the function of placenta, will also affects the fetal outcomes. The aim...
Impact of exercise training on peak expiratory flow rate in relation to body mass index – An Indian perspective
Background: Overweight has a considerable impact on spirometry variables which are thought to be improvised by isotonic exercise and diet regime – though the combined effects have not been investigated and precisely prov...
Variations in the configuration of the posterior cerebral circulation in the adult human cadavers
Introduction: The knowledge on the incidence of the different configurations of posterior cerebral circulation of brain is of great value to the neurosurgeons while dealing with the cerebrovascular diseases as the fetal...