Differential expression of transforming growth factor-β1 and HBx enhances hepatitis B virus replication and augments host immune cytokines and chemokines

Journal Title: Annals of Hepatology - Year 2013, Vol 12, Issue 3

Abstract

[b]Background/Aims[/b]. This study investigated how HBV replication and host immune response are effected by reduced expression of TGF-β1 and HBx. [b]Material and methods.[/b] Short interfering RNA (siRNA) knockdown technology has been used to examine the role of TGF-β1 in hepatitis B virus replication. The siTGF-β1 has been transfected along with 1.3mer HBV x-null to investigate the knockdown effect of TGF-β1 on HBV replication and host immune factors. [b]Results[/b]. In this study, we found that diminished expression of TGF-β1 and increased expression of HBx enhances HBV replication several folds. The differential expression of TGF-β1 and HBx also stimulated transcriptional viral replicative intermediate (pgRNA) and secretion of core and ‘e’ antigen at translational level. Consequently, several cytokines such as IL-2, IL-8 and chemokine monocyte- chemoattractant protein (MCP-1) were increased significantly in response to stimulation of HBV replication. In contrast, TNF-α and RANTES mRNA expression increased insignificantly in response to enhanced HBV replication. [b]Conclusions[/b]. We concluded that reduced expression of TGF-β1 together with HBx expression stimulate HBV replication and immune response, although the underlying mechanism of stimulation most likely differs.

Authors and Affiliations

Fahad Almajhdi, Ahmed Al-Qudari, Zahid Hussain

Keywords

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  • EP ID EP78280
  • DOI -
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How To Cite

Fahad Almajhdi, Ahmed Al-Qudari, Zahid Hussain (2013). Differential expression of transforming growth factor-β1 and HBx enhances hepatitis B virus replication and augments host immune cytokines and chemokines. Annals of Hepatology, 12(3), 408-415. https://europub.co.uk/articles/-A-78280