Differential gene expression of bovine intramuscular and subcutaneous preadipocytes during differentiation
Journal Title: Animal Science Papers and Reports - Year 2012, Vol 30, Issue 2
Abstract
The study suggests that Sirtuin 1 (SIRT1) may play an important role in the development of bovine adipose tissue in vivo. To investigate the differential mRNA expression between bovine intramuscular (IM) preadipocytes (BIPs) and subcutaneous (SC) preadipocytes (BSPs), conventional collagenase-based procedures were modified to harvest preadipocytes from the longissimus dorsi muscle and SC adipose tissue of Luxi cattle, respectively. The time-spatial expression of SIRT1 and associated genes, such as forkhead box O1(FoxO1), peroxisome proliferator-activated receptor-γ (PPARγ), insulin-like growth factor-1(IGF-1), glyceraldehydes-3-phosphate dehydrogenase(GAPDH), and leptin, during bovine preadipocyte differentiation were investigated using real-time quantitative PCR. The intracellular triglyceride concentration of BSPs increased more dramatically than that of BIPs during cell differentiation. The expression of SIRT1 and other genes occurred in the BSPs and BIPs during differentiation indicating that different mechanisms underlie the adipogenesis of BSPs and BIPs, and that the BSPs cultured in vitro show a higher differentiation capacity than BIPs.
Authors and Affiliations
Xiaomu Liu, Kun Zang, Enliang Song, Zongshuai Ling, Xiuwen Tan, Guifen Liu, Haijian Cheng, Fahun Wan
Keywords
Related Articles
- EP ID EP70749
- DOI -
- Views 113
- Downloads 0
Effect of forage legumes on feed intake, milk production and milk quality – a review
Literature data from experiments with lactating dairy cows offered silage-based diets was reviewed to evaluate the effects of the grassland legume species Trifolium repens (WC, white clover), Trifolium pratense (RC, red...
Modelling lactation curve for fat to protein ratio in Holstein cows
The objective of this study was to examine seven mathematical models to evaluate their efficiency in describing the lactation curve for fat to protein ratio (FPR) in the first three lactations of Iranian Holsteins. Data...
SRY-negative, 64,XX sex reversal in a Konik Polski horse: a case report
A three-years old Konik Polski (Polish Primitive Horse) individual was diagnosed as having XX sex reversal syndrome on the basis of female karyotype 64,XX, and the presence of two inter-abdominal testes and ambiguous ext...
The effect of cellulose added to straw bedding on turkeys’ welfare and production results
The study was conducted to investigate the effect of addition of cellulose to straw litter on the degree of bedding encrustation as well as on turkeys’ welfare and production results. Birds were kept either on straw mixe...
A novel pig gene, FASTK, differentially expressed in the muscle tissues from Wujin and Large White pigs
The mRNA differential display technique was used to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated an...