Differentiation in a Single-Tube PCR between Leishmania mexicana and Leishmania braziliensis in Clinical Samples
Journal Title: Microbiology Research Journal International - Year 2015, Vol 6, Issue 4
Abstract
Aim: To assess the usefulness of a single-tube PCR end point for differentiating between Leishmania and Viannia subgenera in clinical samples of cutaneous leishmaniasis. Methodology: Impression smears of 65 patients from Campeche State, Mexico with leishmaniasis-like lesions were analyzed by conventional Giemsa staining and a new simple PCR end point with primers named LU-5A designed by [Harris et al.1998] and LME (designed for this study and based on a mini-exon of L.(L) mexicana,GenBankX64317.1). This PCR generates an amplicon of 210 and 270 bp with L. (L) mexicana (MHOM/MX/84/SETGS) and 230 bp with L. (V) braziliensis (MHOM/BR/75/M2903). Results: Out of 65 impression smears of clinical samples 50 were positive according to PCR (76.9%) and 29 were positive according to microscopic observation (44.6%). The primers did not hybridized with human, Trypanosoma cruzi, Staphylococcus aureus, Staphylococcus epidermidis or Pseudomona aeruginosa DNA. Of the positive smears, 84% (42/50) corresponded to L. (L) mexicana and 16% (8/50) to L. (V) braziliensis. Conclusion: A one-step PCR with primers LU-5A and LME improves the diagnostic capacity to differentiate between Leishmania and Viannia subgenera in parallel with conventional tests.
Authors and Affiliations
Victor Monteon, Enrique Quen-Ramirez, Román Diaz-Arce, María Vargas, Ruth López, Lidia Baylon, José L. Rosales
Keywords
Related Articles
- EP ID EP355050
- DOI 10.9734/BMRJ/2015/12418
- Views 51
- Downloads 0
Assessment of Hygienic Effects of Laundering Using Spectrophotometry
Microbial cleanliness of textiles is an important issue in numerous industry sectors where microorganisms may contaminate manufactured goods and constitute a threat for consumers’ health. The most common method of removi...
Profiling Rhizosphere Microbes on the Root of Maize (Zea mays) Planted in an Alfisol for Selection as Plant Growth Promoting Rhizobacteria (PGPR)
Maize (Zea mays L. Merill) root rhizosphere, being a metabolite-enriched niche was profiled with the objective of isolating Plant Growth Promoting Rhizobacteria (PGPR) for bio-fertilizer production. Isolation was carried...
Molecular Identification, Bioactivity Screening and Metabolic Fingerprinting of the Actinomycetes of Chenab River Sediments
Aims: The mortality, morbidity and health cost have been increased due to multiple antimicrobial drug resistance in pathogens across the world. So it is important to discover new active compounds. This study was designed...
Ventilator Associated Pneumonia in a Tertiary Care Hospital: Incidence, Risk Factors and Etiological Agents
Aims: With an escalating mortality rate reaching 50%, ventilator associated pneumonia (VAP) continues to pose an enormous threat to ICU patients worldwide. Study Design: Prospective cohort study. Place and Duration: T...
Evaluation of Soluble Inducible T cell Co stimulator (sICOS) as a Prognostic Biomarker for Patients with Chronic Hepatitis C Virus
Aim of the Work: This study aimed to investigate the serum level of sICOS molecule in chronic Hepatitis C Virus (HCV) patients and its association with pathological injury and HCV viral load in chronic hepatitis C patien...