Direct detection and identification of mycobacteria in bovine samples by PCR assay
Journal Title: UNKNOWN - Year 2016, Vol 23, Issue 2
Abstract
Bovine tuberculosis, caused by Mycobacterium bovis, is a zoonotic disease causing approximately 6% of total human deaths. Its economic losses are not only a reduction of 10-20% in milk production and weight, but also infertility and condemnation of meat. Many serological tests are applied for detection of tuberculosis. ELISA test has the highest sensitivity and specificity than the other serological tests for the diagnosis of tuberculosis. Several forms of new technology were brought into the diagnostic approach to mycobacterial infection. The aim of this work was to detect bovine tuberculosis by application of different traditional tests and PCR. Tuberculin skin test was applied on 2650 cattle, only 63(2.4%) were positive. Forty eight (76.2%) of the slaughtered positive animals showed visible lesions (VL) while the other 15 (23.8%) had non-visible lesions (NVL). Bacteriological examination of 10 selected tuberculin positive samples revealed M. bovis from 6 processed samples (60%) while PCR and ELISA assays revealed positive in 8 cases (80%) and 7 cases (70%), respectively. It was concluded that PCR test is more sensitive and specific test to confirm the infection with tuberculosis.
Authors and Affiliations
Walid Hamdy Hassan a,*, Essam Amin Nasr b, Hassan Mohamed Moussa c a Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 6511, Egypt. b Department of Tuberculosis, Bacterial Diagnostic Products Veterinary Serum and Vaccine Research Institute, Cairo, Egypt. c General Authorities for Veterinary Services.
Keywords
Related Articles
- EP ID EP97144
- DOI 10.21608/jvmr.2016.43239
- Views 140
- Downloads 0
Evaluation of the bioassay of Commiphora molmol extract (Mirazid) against praziquantel in experimentally infected mice with Schistosoma mansoni
Schistosoma mansoni worms inhabit the portal triad affecting blood elements. Therefore, the current study aimed to compare ameliorative effects of Commiphora molmol extract (Mirazid, MZD) and praziquantel (PZQ) on some...
Prevalence of proteases and other virulence genes in APEC associated with respiratory viral infections in broilers
Acute upper respiratory disease in chickens is a major cause of economic losses due to high mortality rates especially in poorly managed cases. Respiratory disease in poultry is initiated by variety of viruses, bacteria...
Color Doppler ultrasound as an accurate and rapid tool for early pregnancy diagnosis in buffaloes
The objective was to determine the accuracy of color Doppler ultrasound for diagnosis of early pregnancy in buffaloes based on the evaluation of corpus luteum blood flow (CLBF) on days 20 and 21 after mating. Local Egy...
Prevalence of Campylobacter species in milk and some dairy products
Campylobacteriosis is assumed to be mainly a food-borne disease. Also the importance of milk as a source of human Campylobacter enteritis was confirmed by the European Union summary report on food-borne disease outbrea...
Antioxidant, immunostimulant and renal protective activities of tri-herbal combination in African Sharptooth Catfish, Clarias gariepinus
Medicinal herbal feed that used as feed additives are widely applied in livestock production and may be applicable to aquaculture production systems. The present study explores the modulatory effects of incorporation o...