Direct regeneration from leaves and nodes explants of Physalis peruviana L.
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 9
Abstract
Physalis peruviana L. is a popular folk medicine used for treating cancer, leukemia, hepatitis, rheumatism and other diseases. An experiment was conducted to study the effect of growth regulators on plant regeneration in P. peruviana. In this experiment, rhizogenesis and organogenesis were initiated from the leaves and nodes of P. peruviana. The medium used for this purpose was MS with different concentrations of Kin (0, 1, 2 mg/l) and BAP (0, 1, 2, 3, 4 mg/l) for leaves explants and BAP and Kin in combination with different concentration of IBA (0.5, 1 mg/l) for nodal segments. In this study, our objectives were to development of an efficient micropropagation protocol for the medicinal plant Physalis peruviana by the in vitro culture of leaf and nodes explants. The results showed that highest regeneration from leaf and nodal explants was observed at the highest concentration of BAP and Kin. Maximum callus induction (100%) was observed from nodal segment at lower concentration of cytokinine and presence of IBA. Full strength MS solid medium with 1.0 mg/l IBA without or at lower concentrations of cytokinine exhibited the best in vitro rooting.
Authors and Affiliations
Mahmoud Otroshy , Arash Mokhtari, Sayyed Mohammad Mehdi Khodaee and Amir-Hossein Bazrafshan
Keywords
Related Articles
- EP ID EP32290
- DOI -
- Views 422
- Downloads 0
Effect of improvement of soil's physical and chemical characteristics on quality of potato
Potato is an important global food source and one of the most important sources of starch. Interaction between physical and chemical characteristic is important source of variation in any crop including potato, so in t...
The nature and origin of sand dunes in Kashan ERG, Iran
Wind erosion is a process of wind-forced movement of soil particles. This process has the distinct phases of particle entrainment, transport and deposition. Because Iran locate in area that is sensitive about geologica...
Evaluating resistance to drought stress in fluecured tobacco varieties via stress susceptibility indexes in dry farming condition
The evaluate of resistance indices to post anthesis water deficit can help to identify strategies for selection of resistant cultivars of Tobacco (Nicotiana tabacum L.) and increased crop yield production. an experimen...
The effect of high frequency electromagnetic wave (Mobile phone) on Proline aa content in Zea mays seedling
n recent decades, human natural environment undergoes dramatically change due to electromagnetic and magnetic artificial fields increasing and expanded range. The field’s daily functions in home appliance consumptions...
Investigation of gamma irradiation on morphological characteristics and antagonist potential of Trichoderma viride against M. phaseolina
Trichoderma viride was isolated from soil and its spore suspensions were irradiated in different dose of γ-radiation (0-400 Gy) for induced mutation. Dose of 250 Gy γ-radiation approximately results in 43.4% The inhibi...