Direct regeneration from leaves and nodes explants of Physalis peruviana L.
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 9
Abstract
Physalis peruviana L. is a popular folk medicine used for treating cancer, leukemia, hepatitis, rheumatism and other diseases. An experiment was conducted to study the effect of growth regulators on plant regeneration in P. peruviana. In this experiment, rhizogenesis and organogenesis were initiated from the leaves and nodes of P. peruviana. The medium used for this purpose was MS with different concentrations of Kin (0, 1, 2 mg/l) and BAP (0, 1, 2, 3, 4 mg/l) for leaves explants and BAP and Kin in combination with different concentration of IBA (0.5, 1 mg/l) for nodal segments. In this study, our objectives were to development of an efficient micropropagation protocol for the medicinal plant Physalis peruviana by the in vitro culture of leaf and nodes explants. The results showed that highest regeneration from leaf and nodal explants was observed at the highest concentration of BAP and Kin. Maximum callus induction (100%) was observed from nodal segment at lower concentration of cytokinine and presence of IBA. Full strength MS solid medium with 1.0 mg/l IBA without or at lower concentrations of cytokinine exhibited the best in vitro rooting.
Authors and Affiliations
Mahmoud Otroshy , Arash Mokhtari, Sayyed Mohammad Mehdi Khodaee and Amir-Hossein Bazrafshan
Keywords
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