Direct reporting cefazolin from VITEK 2 for E. coli, K. pneumoniae and P. mirabilis isolated from urine cultures using new CLSI interpretations
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2016, Vol 2, Issue 2
Abstract
Background: In recent years, Clinical and Laboratory Standard Institute (CLSI) has recommended a series breakpoint changes for cefazolin, including testing it as a surrogate agent for oral cephalosporins for treating uncomplicated urinary tract infections (uUTIs). Objectives: This study was conducted to evaluate the feasibility of direct reporting the cefazolin results from VITEK 2 for E. coli, K. pneumoniae, K. oxytoca and P. mirabilis isolated from patients with uUTIs using 2014 CLSI recommendation. Material and Methods: Cefazolin susceptibility results of urine cultures of the above four species generated from January 1, 2013 December 31, 2013, using both GN AST card N208 on VITEK 2 and cefazolin disk (gold standard) methods, were extracted from SoftMic Laboratory Information System and analyzed for their category agreement using 2014 CLSI interpretations. Results: Cefazolin susceptibilities of 1969 urinary isolates (1869 patients) of E. coli, K. pneumoniae/K. oxytoca and P. mirabilis comparing their VITEK 2 and disk test results, category agreement for cefazolin tested with both methods was 98%. The linear correlation between sensitive cefazolin and sensitive cephalothin MICs versus cefazolin zone diameters was good, with a predictive value of 99%. Conclusion: It is acceptable to report cefazolin directly from VITEK 2 for the named species from urine cultures. The susceptibility correlation among cefazolin, cephalothin and cefixime were excellent (excluded non-susceptible), further testing with individual oral cephalosporin agents, in this institute, may not be necessary. Recommendation: Final report accompanied by a comment in accordance with 2014 CLSI guideline is recommended to provide therapeutic guidance to clinicians.
Authors and Affiliations
Jianhui Xiong, Bradley Langford, Sigmund Krajden, Zafar Hussain, David Hancock, Mark Downing, William Chapman
Evaluation of the efficiency of the various techniques used for screening of HIV in antenatal mothers to facilitate early initiation of treatment
The study has been conducted in pregnant women in the high risk category attending the antenatal OP at MNR Hospital and medical college and Niloufer Hospital for a defined period The screening tests for HIV used are Coom...
Changing Trends of MRSA Isolation in Tertiary Care Hospital
Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of nosocomial infections worldwide. The aim of study is early detection of MRSA and antibiotic sensitivity pattern. Early detection of MRSA is nece...
Antimicrobial profile of inducible clindamycin resistant strains of staphylococcus species
Introduction The appearance of continuous resistant to multiple drugs among Staphylococci is a global burden due to its ability to cause severe infections The selective use of drugs is necessary to overwhelm the situatio...
Evaluation of anticancer activity of Melaleuka Alternifolia. (i. e. tea tree oil) on Breast cancer cell line (MDA MB)- An in-vitro study
Introduction and Aims According to Globocan 2012 India along with United States and China collectively accounts for almost one third of the global breast cancer burden India is facing challenging situation due to 1154 in...
Seroprevalence of chikungunya fever in a tertiary care hospital in North Karnataka
Introduction Chikungunya disease is caused by Chikungunya virus an Alpha virus under the family Togaviridae transmitted through the vector Aedes spp mosquitoes The sudden onset of very high fever along with rash and seve...