Discovery and Quantification in Mass Spectrometry-Based Proteomics
Journal Title: Journal of Proteomics and Genomics Research - Year 2014, Vol 1, Issue 3
Abstract
Mass spectrometry (MS) has been successfully used to analyze biological samples and advances of MS-based approaches have turn MS data from largely qualitative to quantitative. These MS-based quantitative approaches using label-free, tags, or stable isotope labeling have their own strengths and limitations. The variability introduced by different methods prior to quantitative mass spectrometry should be considered, and accuracy and precision of MS measurements can also vary depending on the strategy used for MS quantification. Therefore, the development of methods for accurate protein quantitation is one of the most challenging areas of proteomics. Using these quantitative approaches, one can investigate the dynamics of proteome through differential protein expression in normal biological processes and diseases.
Authors and Affiliations
Cristian Ruse, Emily I. Chen
Keywords
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- EP ID EP263125
- DOI 10.14302/issn.2326-0793.jpgr-13-357
- Views 115
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