Distribution and molecular basis of A2 subtype in Guangzhou blood donors
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 8
Abstract
Objective To investigate the distribution and molecular basis of A2 subtype among blood donors in Guangzhou. Methods Whole blood samples of 793 group A blood donors in Guangzhou Blood Center were randomly collected. A2 subtype was screened by anti-A1 lectin in 96-well plate, and ABO typing was confirmed by the tube test. Then, the genomic DNA of A2 subtype blood donors was extracted, and the exons 6-7 of ABO gene were sequenced to determine the genotype of A2 subtype. Results Among 793 group A blood donors, there were 13 cases of A2 subtype, and the frequency in group A was 1.64%(13/793). The sequencing results of exons 6-7 of ABO gene showed that 10 of the 13 A2 subtypes carried A2 alleles, which were ABO*A2.05/ ABO*O.01.01 (n=5), ABO*A2.05/ ABO*O.01.02(n=2), ABO*A2.01/ABO*O.01.01(n=1) and ABO*A2.01/ ABO*O.01.02(n=2), respectively. The other 3 cases only carried ABO*A1.02 alleles. Conclusion The main genotype of A2 subtype in Guangzhou is ABO*A2.05, followed by ABO*A2.01.
Authors and Affiliations
Kejun HUANG, Ling WEI, Guangping LUO, Chong CHEN
Keywords
Related Articles
- EP ID EP738321
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.08.014
- Views 3
- Downloads 0
Clinical application progress of platelet-rich plasma in facial aesthetics
Platelet-rich plasma (PRP) is a kind of autologous blood product. It is a platelet concentrate extracted from autologous blood through centrifugation or apheresis process. It is generally believed that the platelet conce...
Influence of blood donation before pregnancy on neonatal birth weight
Objective To explore the influence of maternal blood donation before pregnancy on neonatal birth weight. Methods A total of 6 428 full-term (gestational age ≥37 weeks) singleton pregnant women in Ningbo Medical Center Li...
Performance verification of domestic nucleic acid testing system in blood stations based on T/CSBT
Objective To explore the performance verification of the Shengxiang automatic NAT system for HBV DNA, HCV RNA and HIV RNA-1 using PCR-fluorescence in the laboratories of blood stations, in order to meet the requirements...
The challenge of finding Chinese blood groups
Up to now, hundreds of human blood groups have been detected globally, but none have been found in the Chinese population. China is a multi-ethnic country with rich genetic polymorphism and variation. The Chinese pangeno...
Study on RHD and RHCE gene mRNA based on three generation sequencing technology
Objective To establish RH gene mRNA sequencing method based on nanopores sequencing and to explore the RHD and RHCE mRNA transcripts in D positive and Del individuals. Methods From March 2021 to May 2022, 5 RhD positive...