Distribution and molecular basis of A2 subtype in Guangzhou blood donors
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 8
Abstract
Objective To investigate the distribution and molecular basis of A2 subtype among blood donors in Guangzhou. Methods Whole blood samples of 793 group A blood donors in Guangzhou Blood Center were randomly collected. A2 subtype was screened by anti-A1 lectin in 96-well plate, and ABO typing was confirmed by the tube test. Then, the genomic DNA of A2 subtype blood donors was extracted, and the exons 6-7 of ABO gene were sequenced to determine the genotype of A2 subtype. Results Among 793 group A blood donors, there were 13 cases of A2 subtype, and the frequency in group A was 1.64%(13/793). The sequencing results of exons 6-7 of ABO gene showed that 10 of the 13 A2 subtypes carried A2 alleles, which were ABO*A2.05/ ABO*O.01.01 (n=5), ABO*A2.05/ ABO*O.01.02(n=2), ABO*A2.01/ABO*O.01.01(n=1) and ABO*A2.01/ ABO*O.01.02(n=2), respectively. The other 3 cases only carried ABO*A1.02 alleles. Conclusion The main genotype of A2 subtype in Guangzhou is ABO*A2.05, followed by ABO*A2.01.
Authors and Affiliations
Kejun HUANG, Ling WEI, Guangping LUO, Chong CHEN
Keywords
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- EP ID EP738321
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.08.014
- Views 23
- Downloads 0
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