Distribution of antimicrobial resistance and some widespread extended-spectrum beta-lactamase genes in different phylogroups of Shiga toxin-producing Escherichia coli (STEC) isolates of ruminant origin
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2023, Vol 15, Issue 1
Abstract
Limited data is available on the prevalence of extended-spectrum beta-lactamase (ESBL) genes in Shiga toxin-producing Escherichia coli (STEC) isolates of ruminant origin. This study determined the molecular prevalence of ESBL-encoding genes (blaCTX-M, blaTEM, blaSHV and blaOXA) and antimicrobial-resistance (AMR) of 58 STEC isolates recovered from cattle (n= 32), sheep and goats (n= 26). In the current study, ESBL genes were identified by the molecular technique, while phenotypic AMR against six antibiotics (amoxicillin-clavulanic acid, tetracycline, neomycin, florfenicol, enrofloxacin and sulfamethoxazole-trimethoprim) were tested by disc diffusion method. Phylogenetic groups were also determined by a PCR scheme. Isolates were categorized into five phylogroups (A, B1, C, D and E) and B1 was the most prevalent phylogenetic group (43; 74.1%). Statistical analysis revealed significant association between phylogroup D and small ruminants (sheep and goats, p= 0.014). Moreover, the highest rates of antimicrobial resistance were related to tetracycline (25.9%) and neomycin (22.4%). Resistant isolates to tetracycline (p= 0.001), trimethoprim-sulfamethoxazole (p= 0.013) and neomycin (p= 0.000) were significantly prevalent among strains recovered from cattle. In addition, the majority of MDR strains were also had a significant distribution among cattle isolates (p= 0.001). In the current study, prevalence of ESBL positive STEC was 12.06% (7/58). Genes blaCTX-M and blaTEM were detected separately and in combination in bovine isolates. However, only one STEC strain of small ruminants harbored blaTEM. In conclusion, it seems that cattle isolates are notable sources of different AMR traits which could be a threat to veterinary sections, public health and food hygiene, in particular.
Authors and Affiliations
Rwida Tomeh,Mahdi Askari Badouei,Gholamreza Hashemi Tabar,Hamideh Kalateh Rahmani,
Keywords
Related Articles
- EP ID EP722344
- DOI -
- Views 57
- Downloads 1
A case report of atypical borreliosis in a dog
Borreliosis is a bacterial zoonosis transmitted by arthropods. The infection is more common in dogs than other domestic animals. A one-month-old puppy with fever, jaundice and digestion disorder was referred to Small Ani...
NetB negative Clostridium perfringens infection associated with acute necrotic enteritis in mynah (Acridotheres tristis), grey partridge (Perdix perdix) and turkey (Meleagris gallopavo)
A nonâenterotoxin (CPE)âproducing Clostridium perfringens type A, associated with enteritis in a mynah (Acridotheres tristis), a grey partridge (Perdix perdix) and a turkey (Meleagris gallopavo) was characterized fro...
In vitro evaluation of acaricidal activity of aqueous ozone against Dermanyssus gallina
The aim of this study was to investigate the in vitro acaricidal effect of aqueous ozone against Dermanyssus gallinae (Acari: Dermanyssidae) under laboratory conditions. For this purpose, aqueous ozone at concentrations...
The effect of Artemisinin on the Pentylentetrazole-induced seizures during the estrous cycle and GABA interaction in mice
Catamenial epilepsy may involve 10 to 70% of women with epilepsy in which, seizures are exacerbated by the menstrual cycle. Artemisinin is a herbal compound with widespread modern and traditional medical indications. Bec...
Salvia verticillata Improved Cognitive Deficits in a Chronic Cerebral Hypoperfusion Rat Model
CCH, resulting from multiple cerebrovascular diseases, has been considered the primary cause of cognitive impairment in recent years. In this process, oxidative stress plays a critical role and damages hippocampal neuron...