Distribution of polyethylenimine in zebrafish embryos
Journal Title: Roczniki Państwowego Zakładu Higieny - Year 2018, Vol 69, Issue 3
Abstract
Background. Polyethylenimine (PEI) plays important roles in the pharmaceutical design of non-viral gene delivery systems. Due to a set of unique physicochemical properties this cationic polymer has a great potential in modern gene therapies. Objective. The aim of the present study was to determine the distribution of branched PEI (0.8 kDa) in zebrafish embryos (Danio rerio). Material and methods. Zebrafish embryos at 3 hours post-fertilization (hpf) were incubated with PEI (10 μg/ml) for 24 and 48 hours and studied using the confocal laser microscopy. Results. The obtained results show that PEI effectively distributed into the layers of the chorion and yolk sac in developing embryos due to first 24 hours of exposure. In contrast, PEI was found in the yolk, head, trunk and tail of the embryos due to prolonged treatments (48 hours). Conclusion. The study evidences a high distribution of the branched PEI (0.8 kDa) polymer in the zebrafish embryo tissues.
Authors and Affiliations
Róża Chmielewska, Maciej Gawlak, Magdalena Bamburowicz-Klimkowska, Ireneusz P. Grudziński
Keywords
Related Articles
- EP ID EP497018
- DOI -
- Views 72
- Downloads 0
Diabetic nephropathy in the elderly – clinical practice
Common health problems of the elderly in the near future will become even more common with aging of the population and longer average life expectancy. The elderly tend to have multiple disorders at one time, some of whic...
Migration studies of nickel and chromium from ceramic and glass tableware into food simulants
Background. In addition to the release of lead and cadmium from ceramic and glass vessels, (acceptable limits being set by the EU 84/500/EC Directive), other harmful metals can migrate, such as nickel and chromium. Permi...
Nutrition knowledge of people with eating disorders.
Background. Eating disorders are an increasingly common health problem that is a major therapeutic challenge. For many years, the basic form of therapy used to be psychiatric and psychotherapeutic treatment, but now it i...
Risk assessment for pesticides’ MRL non-compliances in Poland in the years 2011-2015
Background. Human exposure to trace levels of pesticide residues present in food of plant origin is inevitable as long as pesticides continue to be applied in agriculture. Since Maximum Residue Levels (MRL) are not toxic...
Association between blood cholinesterase activity, organophosphate pesticide residues on hands, and health effects among chili farmers in Ubon Ratchathani Province, northeastern Thailand
Background. Use of pesticides has been documented to lead to several adverse health effects. Farmers are likely to be exposed to pesticides through dermal exposure as a result of mixing, loading, and spraying. Organophos...