DISTRIBUTION OF THE CONTENTS OF ACTIVE COMPONENTS IN RADIX FALLOPIAE MULTIFLORAE IN VIETNAM
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2018, Vol 4, Issue 12
Abstract
Radix Fallopiae multiflorae (RFM) is widely used in Vietnam. In this study, high performance liquid chromatography (HPLC) coupled with UV detector has been used for analysis of one stilbene 2,3,5,4′- tetrahydroxystilbene-2-O-β-D-glucopyranoside (THSG) (1) and two anthraquinones [emodin (2), physcion (3)] in RFM. Chromatographic separation was performed on a C18 column (250 x 4.6 mm, 5 μm particle). The mobile phase was a gradient prepared from acetonitrile and 0.5% (v/v) aqueous formic acid. The detection wavelength at 254 nm for EM and PS, 320 nm for THSG. Calibration plots were linear between 15 – 240 μg/mL for THSG, 15.6 – 700 μg/mL for emodin, 43.75 – 72.00 μg/mL for physcion with regression coefficients (R2) > 0.999. The overall recovery was 95.0 – 105.0%. RSD for intra- and inter-day of three analytes (1-3) were less than 3%. The LODs for the determinations of three analytes (1-3) were 0.01 μg/g, 0.005 μg/g, 0.01 μg/mg, respectively. The validated method was successfully applied for quantification of three compounds (1-3) in RFM samples from different locations of Vietnam.
Authors and Affiliations
Nguyen Thi Ha Ly
Keywords
Related Articles
- EP ID EP656534
- DOI -
- Views 180
- Downloads 0
INSOMNIA- AN INCREASING TREND IN PRESENT ERA
Sleep is a natural phenomenon of giving adequate rest to the body and mind. Insomnia is the inability to fall asleep at night for many hours or inability to have a sound sleep, which deprives one of natural rest and inte...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF FLUOROMETHOLONE USING C8 COLUMN IN PARENTERAL DOSAGE FORM BY UPLC TECHNOLOGY
A specific, precise, accurate UPLC method is validated for estimation of Fluorometholone in parenteral dosage form. The method employed, with C8 column (250 ×4.6 mm id)—ACE Generix in gradient mode, with mobile phase of...
WITHANIA SOMNIFERA - THE ANTI STRESS HERB – A REVIEW
According to various surveys, the stress is the major problem for many diseases ranging from psychiatric disorders to endocrine disorders including diabetes mellitus, hypothyroidism, male sexual dysfunction, peptic ulcer...
BACTERIAL CONTAMINATION ON HOSPITAL SURFACES IN BINGHAM UNIVERSITY TEACHING HOSPITAL JOS, NORTH CENTRAL NIGERIA
Background: Frequently touched surfaces in hospital environment can constitute reservoir for healthcare associated infections (HAIS). However, the spread of HAIS involves contacts of susceptible hosts with particularly h...
CIPROFLOXACIN INDUCED FIXED DRUG ERUPTION: A CASE STUDY
Fixed drug eruption (FDE) is a distinctive type of cutaneous drug reaction that characteristically recurs in the same locations upon reexposure to the offending drug. FDEs are less common than exanthematous (morbilliform...