Diversity and phylogenetic analysis of pseudomonas strains isolated from Mingyong glacier, China
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2016, Vol 9, Issue 4
Abstract
On the planet genus of Pseudomonas encompasses debatably the most varied and ecologically important group of bacteria. In all of the main natural environments (Fresh-water, marine and terrestrial) members of Pseudomonas family are found in great numbers and these members also establish intimate relations with animals and plants. This universal spread and distribution represents a notable degree of genetic and physiological adaptability. Within the genus physiological traits do not limit diversity. The variety of phenotypes is also revealed at the genetic level, and evidence is increasing to advocate that the diversity of genome architecture of both the chromosomes and accessory genetic elements is of certain importance. In the present study different Pseudomonas strains were isolated from mingyong glacier. Phylogenetic analysis of 16S rRNA was done and 16S rRNA structures were applied to examine the diversity of Pseudomonas strains. Phylogenetical analysis showed that four branches are formed on the phylogenetic tree. These branches included Pseudomonas strains MY1402, MY1403, MY1408, MY1410, MY0503, MY1412, MY1416, MY1420 and MY1405 which were 9899.9% close to 11 16S rRNA gene sequences of Pseudomonas sp (NCBI GenBank). These four groups can be easily distinguished by comparing their special characteristics in 16S rRNA secondary structures and sequences. The comparison of the 16S rRNA secondary structures of the strains showed that the Pseudomonas strains from mingyong glacier have diversity than other glaciers around the world.
Authors and Affiliations
Muhammad Kamran Taj, Xiuling Ji, Lianbing Lin, Qi Zhang, Yunlin Wei, Imran Taj, Zahoor Ahmed, Ajaz-Ul-Haq, Ghulam Mohammad, Sana Arif, Ashfaq Ahmed
Review of environmental toxins polls in mortality or canola cabbage aphid (Brevicoryne brassicae) in Golestan Province
Rapeseed aphid Brevicoryne brassicae among the key pests of canola in Iran is considered the direct and indirect losses caused by plant diseases and viruses are transmitted. In this study the sensitivity of these aphids...
Evaluation of pearl mussels diversity in Terai region of Eastern Himalaya, India
The present study was conducted to evaluate and generate a primary database on bivalve diversity found in the water bodies of Terai region of Eastern Himalaya, West Bengal, India. A total of 20 species belonging to two g...
The role of quaternary ammonium compound and reducing agent in environmentally friendly alkali treatment of PET /cotton fabric
An environmentally friendly pad-steam process for treatment of polyester/cotton fabric with sodium hydroxide was developed. To enhance the process efficiency, a quaternary ammonium compound (QAC) besides a reducing age...
Allelopathic effects of bermuda grass (Cynodon dactylon L. Pers.) extract on germination and seedling growth of basil (Ocimum basilicum L.) and Common purslane (Portulaca oleracea L.)
Allelopathy is a natural phenomenon that refers to any direct or indirect positive or negative effect of one plant on another through the release of chemical compounds excreted into the environment. In order to evaluate...
Soil and Vegetation Structure in Reclamation Area of Post-Mining Coal, Banjar Regency, South Kalimantan
This research was aimed to assess the soil and vegetation structure in reclamation post-mining coal area of Banjar Regency, South Kalimantan, Indonesia. This study was conducted in ex-mining coal site of Regional Govern...