Diversity of IgG autoantibodies of 4 Chinese intravenous immunoglobulins
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 12
Abstract
Objective To develop methods to display the IgG autoantibody repertoire of intravenous immunoglobulin (IVIG) products, analyze the different types of antibodies and study the diversity of IgG autoantibody in 4 IVIG preparations from different Chinese manufacturers. Methods Two-dimensional gel electrophoresis and immunoblotting with human umbilical vein endothelial cell (HUVEC) proteins were used to demonstrate the IgG autoantibody repertoire and the human protein microarray with bioinformatics analysis was employed to profile the immune reactive autoantigens of the 4 IVIG preparations. Results The methods to showcase the autoantibody repertoire and study the antibody diversity of IVIG were successfully established. High-quality repertoires of IVIG autoantibodies and biological information about self-proteins that can be recognized were obtained. There was a significant difference in the recognition of the quantity and variety of the self-antigens by different IVIG products. The number of antibodies against HUVEC proteins in four products ranged from 241-386. The number of proteins recognized on the human protein chip ranged from 292-435, with 172 human self-proteins recognized by all four products. Conclusion Demonstration of antibody repertoire and protein chip technology can be used to analyze IVIG products′ IgG autoantibody repertoire. All four preparations tested in this study exhibited a broad spectrum of antibodies against HUVEC proteins and human proteome microarray, each product had its unique antibody repertoire characteristics.
Authors and Affiliations
Xiaochen YAN, Li MA, Zongkui WANG, Changqing LI, Tingting LEI, Xi DU, Shengliang YE
Keywords
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- EP ID EP738714
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.12.005
- Views 42
- Downloads 0
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