DNA Sequence Characteristics and Phylogenetics of Three Oligonucleotides Markers on Clariid Species
Journal Title: Biotechnology Journal International - Year 2016, Vol 11, Issue 1
Abstract
Aims: The aim of this study is to express the profiles of three oligonucleotide markers corresponded to reproductive genes that may be different between the two Clariid species (Clarias gariepinus and Heterobranchus bidorsalis) and their phylogenetics. Methodology: Total DNA isolation was carried out on the whole blood of the two strains of Clariid species – 100 species Clarias gariepinus male and female (1.2 – 1.5 kg, 34 – 52 cm) separately; 100 species Heterobranchus bidorsalis (1.7 – 2.2 kg, 38 – 60 cm) respectively using the Quick-gDNA Zymo research kit. Having ascertained the DNA stability on 0.8% agarose gel, NCBI database and Clustal analyses were employed to design primers to reproductive genes that may be different between the two catfishes and may participate in their differential reproducibility. We have used quantitative real-time PCR to investigate the expression of three selected oligonucleotides markers on the catfish. CLC Sequence viewer 7 software was used to analyze the nucleotide alignment percentage and develop the phylogenetics tree. Place and Duration of Study: The study was carried out in the Biotechnology Centre, Federal University of Agriculture, Abeokuta Nigeria between January and July 2014. Results: We observed a dimorphic expression pattern of the three marker genes in relation to strains and sex differentiation, indicating that sox9a retained its function in testis, Figα was highly expressed in the female and Cyp19a1b was up-regulated in male C. gariepinus than male H. bidorsalis catfish species. The phylogenetic tree showed that male Heterobranchus and female Clarias were closer irrespective of male or female while male Clarias differed from the two. Conclusion: To date, these three genes, Sox 9a, Figα and Cyp19a1b have been detected in many fishes, but little or no data has been reported in African catfishes. The findings from this study might be used as the target gene for catfish gender regulation.
Authors and Affiliations
O. T. Agbebi, I. Abduraheem
Keywords
Related Articles
- EP ID EP237716
- DOI 10.9734/BBJ/2016/21589
- Views 102
- Downloads 0
Isolation and Identification of Actinomycetes from Mangrove Soil and Extraction of Secondary Metabolites for Antibacterial Activity
The mangrove ecosystem of India is an extensively unexplored source for actinomycetes with the potential to produce secondary metabolites of biological importance. In this study, twenty two actinomycetes were isolated fr...
Optimisation of Growth Factors for Effective Use of Phosphate Solubilizing Bacterial Strains and Its Use as Bioinoculants for the Growth of Groundnut (Arachis hypogaea) Plant
Phosphorus (P) is one of the essential macronutrients for plant growth. Phosphate solubilizing bacteria (PSB) are organisms which are used as bioinoculants to enhance the plant growth. Plants take phosphate in the form o...
Agrobacterium-Mediated Transfоrmation of Maize with Antisense Suppression of the Proline Dehydrogenase Gene by an In Planta Method
Aims: The aim of this study was to estimate the efficiency of T-DNA transfer during Agrobacterium-mediated transformation of maize (Zea mays L.) at different temperatures. In addition, the way of T-DNA transfer was studi...
In vitro Cytological Studies of Leaf Callus Cultures of Orthosiphon aristatus (Blume) Miq
The study was carried out to unravel the in vitro cytological behaviour of leaf callus cultures of Orthosiphon ariststus (Blume) Miq. The cultures were raised using leaf explants on Murashige and Skoog (MS) medium supple...
Prevalence and Antibiotic Susceptibility Pattern of Methicillin Resistant Staphylococcus aureus in Tertiary Care Hospitals
Aims: Methicillin resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in hospitals with hospital based outbreaks world-wide. The present study was carried out to estimate the prevalence of MRSA isolates...