Donor plant mycobiota and filamentous fungi contaminants from the in vitro establishment of five species forest
Journal Title: Biotecnología Vegetal - Year 2009, Vol 9, Issue 2
Abstract
Although protocols to propagate large numbers of woody species by in vitro culture have been developed, microbial contamination of explants is a fundamental problem which limits its application. The objectives of this study were: to identify the donor plant mycobiota and to identify filamentous fungi contaminants at the establishment phase of mahogany, teak, majagua, pine and cedar. Explants of donor plants were placed in moist chamber and were prepared to direct optical microscope of fungal growth. Cultural characteristics and morphological were taken into account to identify filamentous fungi contaminants in the establishment phase. Frequency of all genera identified was determined. Eleven genera of filamentous fungi in donor plants and twenty genera in the establishment of forest species were identified. The genera Botryodiplodia, Cephalosporium, Diplodia, Dreschlera, Geotrichum, Gloeosporium Helminthosporium, Memnoniella, Oidiodendron and Rhinoclediella appeared only during establishment phase. Others such as Alternaria, Aspergillus, Cercospora, Curvularia, Cladosporium, Colletotricum, Fusarium, Nigrospora, Penicillium and Pestalotia were detected in donor plants and during establishment phase. This indicates that the possible source of contamination was the initial explant. The most frequent genera were Botryodiplodia in teak, Nigrospora in majagua and Alternaria in pine, cedar and mahogany. A phytosanitary plan of defence for donor plants was developed. The plan included systemic and contact fungicides whose spectrum of activity covered the identified genera of fungi.
Authors and Affiliations
M. Acosta-Suárez, Y. Alvarado-Capó, M. Cruz-Martín, M. Leiva-Mora, C. Sánchez-García, Berkis Roque, E. Quiala, Maité Chávez, F. Jiménez-Terry, Mariana la O, R. Barbón, R. Collado, Mayelín Rodríguez, M. De Feria, Ivan Borroto, Martha Pérez
Keywords
Related Articles
- EP ID EP683857
- DOI -
- Views 132
- Downloads 0
Use of AFLP markers for molecular characterization of two crops with agricultural interest
The AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction (PCR) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA),...
Protocol for the production of Xanthosoma minitubers sagittifolium (L.) Schott. at home growing from plants in vitro
The use of biotechnological techniques for Xanthosoma saggitifolium (L.) Schott propagation and conservation allows using plant material with high genetic and health qualities. However, production volumes of taro seeds...
Predicting the affinity of A 2A adenosine receptor antagonists using decision trees
Neurodegenerative diseases are being treated by modulating adenosine receptors with more effective, safe and selective antagonists. The objective of the study was to develop a methodology to obtain classification models...
Effect of a propolis formulation on the culture media for potato micropropagation var Desirée
In the efforts to improve the quality of the plants in the in vitro culture, different alternatives have been tested and put into practice going from the increasing of the asepsis measures, chemical treatment of the pla...
Novel explant for somatic embryogenesis in Sorghum bicolor (L.) Mohen
This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-05’ starting from callus obtained from sections of in vitro shoots. For the formation of callus, different concentrations o...