Dosage by High Performance Liquid Chromatography (HPLC) coupled with the dissolution of a combination of antituberculous: a pilot study conducted in Cotonou, Benin
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2015, Vol 3, Issue 4
Abstract
Efficient tuberculosis treatment requires the use of good quality medicines. The present study developed a method of dosage by High Performance Liquid Chromatography (HPLC) coupled with the dissolution of a combination of fixed doses of four anti-tuberculous (Isoniazid, Pyrazinamide, Ethambutol Hydrochlorate, Rifampicine). The elaborated protocol was thereafter used to assess the quality of medicines commonly used in Benin as per the National Program for Tuberculosis Control (PNT). An analytical procedure was developed and validated by statistical tests of Cochran and Grubbs. Five lots of anti-tuberculous were randomly sampled (from India and tuberculosis screening and treatment centres in Benin). These lots were subjected to a number of tests including preliminary, disintegration, identifications and dissolution tests coupled with HPLC and dosages. The established procedure is demonstrated to be applicable with 80% accuracy. Results of the quality control revealed 100% conformity to all the tests for all tested drugs from all lots. The elaborated procedure is therefore applicable, rapid, effective and simple.
Authors and Affiliations
F. Baba-Moussa| Laboratoire National de Contrôle de Qualité des Médicaments et Consommables Médicaux (LNCQ) 06 BP139 Cotonou, BENIN, Laboratoire de Microbiologie et de Technologie Alimentaire, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, ISBA-Champ de foire Cotonou, BENIN, J. Bonou| Laboratoire National de Contrôle de Qualité des Médicaments et Consommables Médicaux (LNCQ) 06 BP139 Cotonou, BENIN, Laboratoire de Microbiologie et de Technologie Alimentaire, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, ISBA-Champ de foire Cotonou, BENIN, H. Ahouandjinou| Laboratoire National de Contrôle de Qualité des Médicaments et Consommables Médicaux (LNCQ) 06 BP139 Cotonou, BENIN, Laboratoire de Microbiologie et de Technologie Alimentaire, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, ISBA-Champ de foire Cotonou, BENIN, V. Dougnon| Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey-Calavi/Université d’AbomeyCalavi, BENIN, G. Houndjo| Laboratoire National de Contrôle de Qualité des Médicaments et Consommables Médicaux (LNCQ) 06 BP139 Cotonou, BENIN, M. Gninafon| Programme National Contre la Tuberculose, Ministère de la Santé, Cotonou, BENIN, F. Gangbo| Laboratoire de Phytogénétique et de Biologie Humaine, ISBA-Champ de foire Cotonou, BENIN, F. Toukourou| Laboratoire de Microbiologie et de Technologie Alimentaire, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, ISBA-Champ de foire Cotonou, BENIN, F. Loko| Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey-Calavi/Université d’AbomeyCalavi, BENIN, I. Aboudoulaye| Laboratoire de Biochimie Faculté des Sciences de la Santé – CNHU-HKM, Cotonou, BENIN, D. Kinde-Gazard| Laboratoire de Parasitologie et de Mycologie, Faculté des Sciences de la Santé/Université d’Abomey-Calavi, Cotonou, BENIN
Keywords
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- EP ID EP13513
- DOI 10.18869/IJABBR.2015.332
- Views 381
- Downloads 14
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