Down-regulatory Influence of Methanol and Aqueous Root Extracts of Sphenocentrum jollyanum on Some Fertility Hormones and the Effect on Testicular Size of Wistar Albino Rats
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 18, Issue 3
Abstract
Objective: The study is aimed at investigating the effects of oral administration of aqueous and methanol extract of the root of Sphenocentrum jollyanum on testicular size, testosterone, follicle- stimulating hormone, luteinizing hormone and prolactin levels in male Wistar albino rats. Methods: A total of 24 Wistar albino rats of equal sexes were randomly distributed into three groups having 4-rats each of both sexes. Group 1 served as the control and was administered distilled water ad libitum while groups 2 and 3 were administered 400 mg/kg body weight methanol and aqueous extracts respectively. Oral administration lasted for 14 days at the end of which animals were sacrificed and blood samples were collected from all the groups through ocular puncture into plain sample bottles for hormonal assay. The serum level of progesterone, estradiol, testosterone, follicle stimulating hormone, luteinizing hormone and prolactin were determined using enzyme-linked immunosorbent assay (ELISA) technique. The testicular weight of the animals was equally determined. Results: There was a non-significant (p˃ 0.05) variation in the mean testicular size of the treated and control rats, although the testicular size of the treated rats increased within the period when compared with the control group. Furthermore the administration of both aqueous and methanol root extracts of S. jollyanum caused non-significant (p>0.05) decrease in estradiol concentration when compared to the control. The levels of progesterone, follicle stimulating hormone, luteinizing hormone and prolactin significantly (p<0.05) decreased when compared to the control in the aqueous-treated group while the methanol extract-treated group showed a non-significant (p>0.05) difference in the luteinizing hormone and prolactin levels while follicle stimulating hormone was significantly (p<0.05) decreased. All comparisons were made against the control group. A significant (p<0.05) decrease in testosterone concentration of the aqueous group and a non-significant (p>0.05) decrease in the methanol extract group was seen in male rats. The result of follicle stimulating hormone level showed a significant (p<0.05) decrease in both aqueous and methanol extracts treated groups while a non-significant (p>0.05) reduction in luteinizing hormone was observed in both extract administered rats. Conclusion: The results of this study showed that the aqueous and methanol root extracts of the S. jollyanum may possess anti-fertility properties in both sexes and can have some implications for fertility and contraception.
Authors and Affiliations
S. I. Egba, H. C. Omeoga, C. E. Oriaku, E. C. Jacob, F. C. Nnabugwu, J. C. Lazarus
Keywords
Related Articles
- EP ID EP313528
- DOI 10.9734/ARRB/2017/36598
- Views 76
- Downloads 0
Assessment of Phytoplankton Diversity in Al-Diwaniya River, Iraq
Background and Aim: The present study was conducted to determine the biodiversity of the phytoplankton community in Al-Diwaniya River. Place and Duration of Study: Al-Diwaniya River in Al-Qadisiyah Province at three stat...
Degradation of Phenol Containing Wastewater by Advance Catalysis System – A Review
Phenols and their derivatives are broadly distributed as a characteristic pollutant due to its frequent presence in effluents of many industrial processes. Most of the phenolic compounds are toxic to living organisms as...
Effects of Muscular Temporomandibular Disorder on Masticatory Muscles’ Thickness
This study analyzed the impact of muscular temporomandibular disorder (TMD) on masseter and temporalis muscles’ thickness. Eighty subjects (aged 21–60, both male and female) with complete dentition were divided into stud...
Physiological Peculiarities of Vessels’ Disaggregating Control over New-Born Calves’ Erythrocytes
Introduction: The process of microcirculation determines significantly the common physiological status of calves including the new-born phase. The success of microcirculation process in tissues mostly depends on aggregat...
In vitro Development of Chimeric Bovine Embryos Created by Combining a Single Blastomere of SCNT Embryos with an IVF Embryo
In this study, we produced chimeric embryos from a single blastomere of somatic cell nuclear transfer (SCNT) bovine embryo and in-vitro fertilized (IVF) bovine embryo, and examined the rate of blastocyst development and...