Drop collapse assay on lotus leaf (Nelumbo nucifera): a simple and cost effective method for rapid detection of biosurfactants
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2016, Vol 4, Issue 0
Abstract
An alternative technique to perform drop collapse assay for rapid detection of surface active agents produced by microorganisms is described. The method is rapid, simple, economical and sensitive as it can detect the biosurfactant even in low concentrations. The limit of detection for Triton-X 100:0.01 mM (6.25 µg mL-1), CTAB: 0.1 mM (36.44 µg mL-1) and SDS: 0.001 mM (0.288 µg mL-1) using lotus leaf assay. This method uses Lotus (Nelumbo nucifera) leaf as the surface for performing drop collapse studies and can be useful as an initial step for screening microorganisms for biosurfactant production and for detection of surfactant activity. The underline principle was that the leaves of lotus are super hydrophobic, i.e. drops of water roll off free of residue. The lotus leaf has surface roughness and posses water-repellent wax crystals which attribute towards super hydrophobic properties. Hence, if surface active agent capable of reducing surface and interfacial tension acting as wetting agent is produced by the Microbispora sp. V2 even in low concentrations, the drop collapse will occur on lotus leaf and wetting of leaf can occur. Lotus leaf can be a better alternative to microtitre plates as due to its rapidity, sensitivity, simplicity, ease, cost effectiveness and reproducibility. The simplicity of this technique makes it suitable in rapid screening of large number of surfactant producing microbes without the need of expensive high throughput systems.
Authors and Affiliations
Meghmala S Waghmode, Neha N Patil, Pallavi S Gaikwad, Aparna B Gunjal, Neelu N Nawani, Balu P Kapadnis
Keywords
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- EP ID EP429683
- DOI 10.18006/2016.4(5).505.511
- Views 63
- Downloads 0
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