Dual Targets in PCR and its Usage for the Molecular Characterization of Mycobacterium Tuberculosis
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2015, Vol 3, Issue 3
Abstract
Tuberculosis (TB) is an infectious bacterial disease caused by Mycobacterium tuberculosis. Diagnostics methods includes Serological, Radiological and Microbiological investigations. The current study was done on the dual targets i.e. IS6110 and mpb-64 for the characterization of tuberculosis in 25 cases with the usage of Nested PCR, and Conventional PCR. 12 patients were negative and 13 were positive for Tuberculosis PCR. Further it was observed that out of 13 positive patients all gave positive target amplification for IS6110 gene whereas only 5 of them were observed positive for mpb64. Thus IS6110 is a better molecular marker for the PCR reaction when used in routine diagnosis. Keywords: Polymerase chain reaction, Nested PCR, Mycobacterium tuberculosis, uracil-N-glycosylase.
Authors and Affiliations
Rachana Panwar, Udita Bharti, Priyanka Bhatt, Ankit Rana, Sheetal Verma, Neelam Negi, Narotam Sharma
Keywords
Related Articles
- EP ID EP374467
- DOI -
- Views 75
- Downloads 0
Adverse drug reaction reporting among clinicians in a teaching hospital in South Karnataka
The study was aimed at investigating knowledge, attitude and practice of clinicians towards ADR reporting, to identify the reasons for underreporting and suggestions to improve the ADR reporting system. A questionnaire s...
An Unexpected Diagnosis of Systemic Lupus Erythematosus (SLE) Four Weeks after Mitral Valve Replacement: the Need for a Deep Understanding
Systemic lupus erythematosus (SLE) is one of the most common autoimmune diseases. Here, we present a special surgical case of mitral stenosis with SLE. Mitral valve replacement was performed, and the postoperative course...
Prevalance of Depression in Patients with ESRD on HD- A Cross Sectional Observational Study in a Teaching Hospital in Karnataka
To assess the prevalence of depression in patients on chronic hemodialysis and to evaluate the risk factors. 100 patients undergoing hemodialysis were included in this study. After obtaining ethical clearance and informe...
Standardization and Validation of Dot ELISA, Finding Detection Limit of Chlorpyrifos in Field Sprayed Samples Comparatively To Indirect Plate ELISA and Gas Chromatography
Abstract: A Dot enzyme linked immonosrbent assay (Dot ELISA) was developed and compared with standard indiect plate ELISA and gas chromatography (GC) for chlorpyrifos detection in the field samples. Firstly Dot ELISA for...
The Association between Oral Health Habits and Quality of Life for Residents of a Psychiatric Nursing Home in Southern Taiwan
Abstract: The issue of daily care for patients with chronic mental disorders has recently been given more consideration. Many studies have revealed a strong association between oral diseases and chronic diseases. There i...