Dual Targets in PCR and its Usage for the Molecular Characterization of Mycobacterium Tuberculosis
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2015, Vol 3, Issue 3
Abstract
Tuberculosis (TB) is an infectious bacterial disease caused by Mycobacterium tuberculosis. Diagnostics methods includes Serological, Radiological and Microbiological investigations. The current study was done on the dual targets i.e. IS6110 and mpb-64 for the characterization of tuberculosis in 25 cases with the usage of Nested PCR, and Conventional PCR. 12 patients were negative and 13 were positive for Tuberculosis PCR. Further it was observed that out of 13 positive patients all gave positive target amplification for IS6110 gene whereas only 5 of them were observed positive for mpb64. Thus IS6110 is a better molecular marker for the PCR reaction when used in routine diagnosis. Keywords: Polymerase chain reaction, Nested PCR, Mycobacterium tuberculosis, uracil-N-glycosylase.
Authors and Affiliations
Rachana Panwar, Udita Bharti, Priyanka Bhatt, Ankit Rana, Sheetal Verma, Neelam Negi, Narotam Sharma
Keywords
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