EFF ECT OF POTASSIUM DICHROMATE IN THE ENVIRONMENT ON BIOCHEMICAL PARAMETERS OF PEA PLANTS
Journal Title: Вісник Одеського національного університету. Біологія - Year 2015, Vol 20, Issue 2
Abstract
The aim of this study was to investigate the intensity of free radical processes in pea plants exposed to different concentrations of potassium dichromate. Pea plants (Pisum sativum L.) were grown on the sand which contain potassium dichromate K2Cr2O7 in concentrations of 0.001, 0.01 and 0.1 mM per kg of substrate. Plants leaves were harvested on the 5th, 10th and 15th days of growth. The concentration of carbonyl protein groups and thiobarbituric acid reactive substance (TBARS), catalase and guaiacole peroxidase activity were measured in apical leaves. The level of protein carbonyls was not changed in the apical leaves of pea as compared with controls in all experimental groups. Pea plants growing in the presence of potassium dichromate resulted to intensification of free radical processes as evidenced by the increased thiobarbituric acid reactive substance. The activity of catalase did not change in leaves of plant exposed to potassium dichromate. Guaiacole peroxidase activity increased by 1.2 and 3.5 fold in plant grown in the presence of 0.01 and 0.1 mM potassium dichromate, respectively as compared to control. It can be suggested that guaiacole peroxidase play an important role in plant protection from potassium dichromate toxicity.
Authors and Affiliations
U. Ya. Stambulska
Keywords
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