Effect Of 0.2% Glutaraldehyde Disinfectants on Adherence of Candida Albicans on Heat Cure Acrylic Resin, Cobalt-Chromium Alloy and Flexible Denture Base Material- An In Vitro Study
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 12
Abstract
Abstract: Objective of this study is to evaluate the effectiveness of 0.2% glutaraldehyde on the adherence of Candida albicans on heat cure acrylic resin, cobalt-chromium alloy and flexible denture base materials.Total 45 samples were made, 15 from each type of denture base material i.e. Heat cure acrylic resin followed by flexible acrylic resin and cobalt chromium alloy. For Candida albicans adherence, all group samples were placed in standardized cell suspension (ATCC 26555) of Candida albicans. Viable counts of the microorganism were determined by colony count. Results were expressed in cfu/mm2.To test the antifungal efficacy of Glutaraldehyde, the procedure were repeated for all the disc samples of the all group where in the disc samples were treated with the disinfectants separately for 12 hrs and tested for Candidal adherence. The mean of CFU/mm2 of C. albicans onheat cure acrylic resin, flexible acrylic resin and cobalt-chromium alloy were 99.83 cfu/mm2, 80.21 cfu/mm2 and 69.90 cfu/mm2 respectively. The mean of CFU/mm2 of C. albicans after treated with 0.2% Glutaraldehyde on heat cure acrylic resin and flexible acrylic resin were 0.47 cfu/mm2 and 0.87 cfu/mm2 respectively.The high candidal retention was observed on Heat cure acrylic resin followed by flexible acrylic resin and cobalt chromium alloy. At 0.2% Glutaraldehyde there were slight growth of c. Albicans on Heat cure and flexible acrylic resin but no growth on Co-Cr alloy material. Keywords:Denture Stomatitis, Denture biofilm, Glutaraldehyde, C.albicans, Denture base material.
Authors and Affiliations
Manish Kumar, Manas Bajpai, Monika Sharma, Dinesh Chand Sharma
Keywords
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