EFFECT OF А PURIFIED SAPONINS’ MIXTURE FROM ASTRAGALUS GLYCYPHYLLOIDES, ADMINISTERED ALONE, ON ISOLATED RAT BRAIN SYNAPTOSOMES AND HEPATOCYTES
Journal Title: TRADITION AND MODERNITY IN VETERINARY MEDICINE - Year 2018, Vol 3, Issue 2
Abstract
Purified saponins’ mixture (PSM), obtained from Astragalus glycyphylloides (family Fabaceae) was examined for possible toxic effects on isolated rat brain synaptosomes and hepatocytes when adminis-tered alone. Synaptosomes and hepatocytes were incubated with PSM at three different concentrations: 60 µg/ml; 6 µg/ml; 0.6 µg/ml. The effects of PSM were compared to those of silymarin, in the same con-centrations. The main parameters, characterizing functional and metabolic status of synaptosomes and hepatocytes were investigated: viability (by Trypan blue – for hepatocytes and MTT-test – for synapto-somes), lactate dehydrogenase activity (LDH), level of reduced glutathione (GSH) and production of malondialdehyde (MDA). It was found that administered alone the PSM did not reveal any statistically significant toxic effects on both isolated rat brain synaptosomes and hepatocytes, compared to silymarin.
Authors and Affiliations
Georgi Popov, Magdalena Kondeva-Burdina, Vasil Manov, Aleksandar Shkondrov, Ilina Krasteva
Keywords
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- EP ID EP421828
- DOI 10.5281/zenodo.1489280
- Views 87
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