Effect of additives on Pleurotus ostreatus Growth on Agar medium
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 3
Abstract
Oyster mushroom (Pleurotus ostreatus) is one of the most widely grown mushrooms worldwide. This type of fungus have been grown for centuries in green houses to produce mycelium and fruiting bodies and marketed as highly nutritious food. Like many other mushrooms, oyster mushroom characterized by lower growth rate which consume long time on agar medium for inoculum preparation for spawn preparation. Therefore, design of new cultivation medium to shorten the cultivation time on solid medium is necessary to reduce time and produce inoculum with less cost. This work was focused on optimization of agar medium (using the conventional potato dextrose agar medium PDA as base medium for growth and supplemented with other components such as malt extract, yeast extract, and sodium nitrates). The results clearly demonstrate that addition of malt extract and yeast extract support dense cell growth but not fast radial growth on agar medium. Addition of sodium nitrate in addition to malt and yeast extract, increase both of radial cell growth and dense growth as well. Therefore, for fast growth of Pleurotus ostreatus on agar medium its recommended to supplement the growth medium with malt extract, yeast extract, and sodium nitrate.
Authors and Affiliations
Parisa Maftoun, Roslinda Abd Malek, Helmi Johari Masry, Farid Agouillal2 ,, Avnish Pareek, Siti Zulaiha Hanapi, Hesham A. El Enshasy1,4,
Keywords
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