EFFECT OF AGROBACTERIUM RHIZOGENESIS ON HAIRY ROOTS INDUCTION IN FENNEL (FOENICULUM VULGARE MILLER)
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Thisstudy was conducted to access the effects of seven Agrobacterium rhizogenes strains i.e. A4, T4, MSU, TCC15834, 2656, Gm1 and R1000, on the hairy root generation from various explants i.e. hypocotyl, cotyledon, root, stem and leaf of Fennel ecotypes (Bonab, Izmir, Esfahan and Moghan). Results of study revealed that induction of hairy root varies with the A. rhizogenes strains and used explants. Among the various tested combinations, highest root induction was recorded form hypocotyl explants using A4 and TCC 15834 (70 and 67%, respectively)strains of A. rhizogenes. Response of explant toward hairy root induction and number of the hairy roots per explant was different and decreased from hypocotyl, leaf and cotyledonary explants, respectively. Among the various tested A. rhizogenes strain, TCC 15834 capable to induced hairy Roots development in all ecotypes but most effectively in Izmir (36.66 ± 0.57) and it was followed by Bonab (32 ± 0.44), Esfahan (30 ± 0.99) and Moghan (16.66 ± 0.79). All transformed hairy root were successfully confirmed by PCR using rolB primers. Further hairy root induction could be proved as an alternative approach for the production of secondary metabolites and its increasing use as model system in metabolic engineering.
Authors and Affiliations
Nishtman abdi, Ebrahim Dorani Uliaie, Ali Bandehagh, Saeed Aharizad
Keywords
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- EP ID EP428034
- DOI 10.18006/2017.5(3).384.391
- Views 102
- Downloads 0
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