Effect of an hydro-alcoholic extract of Uncaria tomentosa (cat´s claw) over dendritic cell subsets and HLA-DR/CD86 molecules by lipopolysacarides stimulus
Journal Title: Revista Peruana de Medicina Experimental y Salud Publica - Year 2009, Vol 26, Issue 2
Abstract
[b]Objective. [/b]To determine the effect of an hydro-alcoholic extract of cat´s claw Uncaria tomentosa (UG) over lipopolysaccharides – treated dendritic cells (DC) and HLA-DR and CD86 molecules expression of peripheral blood samples obtained from healthy individuals. [b]Materials and methods.[/b] Peripheral blood samples were collected from healthy individuals. Peripheral Blood Mononuclear Cells (PBMC) were isolated by centrifugation over density gradient, pre-treated with and without the addition of UG, and then stimulated for 24 h with lipopolysaccharides (LPS), then the cells were labelled with specific fluorescent monoclonal antibodies to HLA-DR, Linage type 1 (Lin1), CD11c and CD86 and prepared for flow. [b]Results.[/b] Compared to PBMC with LPS but without UG group, we found a decrease of myeloid DC (mDC) percentage (p<0.05) and a tendency to increase plasmacytoid DC (pDC) percentage, both in a dose-dependent manner. There was a decrease in media fluorescence intensity (MFI) of HLA-DR in DCp between 500 and 1000 μg/mL of CC (p<0,001), but we found a increase in MFI of CD86 at all concentrations (p<0,05). There was no effect of UG over MFI of the HLA-DR or CD86 expression in mDC. [b]Conclusions. [/b]UG showed a tendency to increase pDC percentage and decreased mDC percentage. UG did not affect the expression of CD86 and HLA-DR from mDC. This study demonstrates that UG promotes activation/differentiation of pDC, which has a demonstrated involvement in the adaptive immune response mechanisms.
Authors and Affiliations
Iván Lozada-Requena, César Núñez, Yubell Álvarez, José Aguilar
Keywords
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