Effect of antioestrogens on proliferation, differentiation and telomerase activity of pig granulosa cells in vitro
Journal Title: Journal of Animal and Feed Sciences - Year 2010, Vol 19, Issue 4
Abstract
The aim of the present work was to study the effect of oestrogen receptor inhibitors (ICI 164.384; ICI and cyclofenil; CF) on proliferation, oestradiol synthesis, aromatase expression and telomerase activity (TA) of pig granulosa cells (GC) derived from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. Cells were treated with anioestrogens for 48 h in basic (medium only) as well as FSH-stimulated conditions. Antioestrogens applied individually and in a combination significantly (P<0.01) decreased proliferative potential of LF-GC. Antioestrogens applied in a combination increased (P<0.05-0.01) 17-β oestradiol synthesis in small and large follicle GC. Antioestrogens applied individually and in a combination caused an increase (P<0.01) of aromatase gene expression in FSH-stimulated conditions as well as in basic conditions. Moreover, antioestrogens increased (P<0.05-0.01) TA in basic and in FSH-stimulated conditions. The results of the study indicate the involvement of oestrogen receptor α and β in the control of proliferation, differentiation and telomerase activity in pig GC. Furthermore, telomerase activity does not have to be linked only to pig GC proliferation but may also be involved in the differentiation process.
Authors and Affiliations
E. Chronowska, M. Tomanek, T. Kott
Keywords
Related Articles
- EP ID EP73859
- DOI -
- Views 123
- Downloads 0
Performance, carcass quality and blood metabolites of Holstein bulls on feedlot feeding of different proportions of barley grain to maize grain
This experiment was conducted to study the effects of five different ratios of barley grain to maize grain: 1) 100:00, 2) 75:25, 3) 50:50, 4) 25:75, and 5) 00:100 on feedlot performance, carcass quality, ruminal and bloo...
The role of animal feeding trials for the nutritional and safety assessment of feeds from genetically modified plants – Present stage and future challenges
The world´s population is still growing up and asking for more and better food. High yielding plants with low external inputs should be the main aim of plant breeding to meet the higher demand for man and animal. Apart f...
Raw vs extruded coloured-flower pea as an ingredient in broiler diets: effects on performance, ileal digestibility, gut morphology, and intestinal microbiota activity
T: Raw or extruded peas (Pisum sativum, pink-flowered cv. Milwa) were used. Extrusion reduced the trypsin inhibitor activity (TIA) from 2.35 to 0.35 mg · g–1, but did not affect tannins content, which was about 1 g · kg–...
Detoxification of aflatoxin B1 and change in microflora pattern by probiotic in vitro fermentation of broiler feed
The aim of this study was to determine the influence of spontaneous fermentation and that with the use of probiotic bacteria and yeast on aflatoxin B1 concentration and the microflora pattern during fermentation. The pr...
An interdisciplinary study on the mode of action of probiotics in pigs
An interdisciplinary research group granted by the German Research Foundation (FOR 438) tested various hypotheses and tired to develop a model for the mode of action of probiotics in pigs. The study included the fields o...