Effect of antioestrogens on proliferation, differentiation and telomerase activity of pig granulosa cells in vitro
Journal Title: Journal of Animal and Feed Sciences - Year 2010, Vol 19, Issue 4
Abstract
The aim of the present work was to study the effect of oestrogen receptor inhibitors (ICI 164.384; ICI and cyclofenil; CF) on proliferation, oestradiol synthesis, aromatase expression and telomerase activity (TA) of pig granulosa cells (GC) derived from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. Cells were treated with anioestrogens for 48 h in basic (medium only) as well as FSH-stimulated conditions. Antioestrogens applied individually and in a combination significantly (P<0.01) decreased proliferative potential of LF-GC. Antioestrogens applied in a combination increased (P<0.05-0.01) 17-β oestradiol synthesis in small and large follicle GC. Antioestrogens applied individually and in a combination caused an increase (P<0.01) of aromatase gene expression in FSH-stimulated conditions as well as in basic conditions. Moreover, antioestrogens increased (P<0.05-0.01) TA in basic and in FSH-stimulated conditions. The results of the study indicate the involvement of oestrogen receptor α and β in the control of proliferation, differentiation and telomerase activity in pig GC. Furthermore, telomerase activity does not have to be linked only to pig GC proliferation but may also be involved in the differentiation process.
Authors and Affiliations
E. Chronowska, M. Tomanek, T. Kott
Keywords
Related Articles
- EP ID EP73859
- DOI -
- Views 97
- Downloads 0
Influence of different dietary oil sources on performance and fatty acid profile of egg yolk in laying hens
Laying hens were fed diets containing sunflower, fish, linseed and rapeseed oil with 2 levels of inclusion (1.5 and 3.0%) for 12 weeks. Egg production, egg weight, feed intake, feed conversion and liveweight were not sig...
Effects of some non-genetic factors on concentration of urea in milk in Polish Holstein-Fresian cows
The objective of this study was to estimate lactation curves of milk urea concentration for primiparous Polish Holstein-Friesian cows, milked in different months and air temperatures, using random regression test-day mod...
The effects of benzoic acid and essential oil compounds in combination with protease on the performance of chickens
T. Experiments were conducted to study the effect of benzoic acid and of essential oil blends in combination with protease on the growth performance of broiler chickens. In the first trial, the birds were divided into th...
A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes
simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed prem...
The effect of dietary yellow lupine (L. luteus cv. Baryt) on growth performance, carcass characteristics, meat quality and selected serum parameters of turkeys
This study evaluated the effects of dietary replacement of soyabean meal (SBM) with yellow lupine seed meal (YLM) in turkeys. A total of 1120 one-day-old Hybrid Converter female turkeys were fed diets containing 0%, 8%,...